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Research background with coronary thrombolysis, coronary bypass techniques become more sophisticated and promote the use and treatment of heart disease is no longer a problem, but the technology behind the long-term there is a growing concern of myocardial ischemia / reperfusion injury problems as well as a series of follow-up due to ischemia / reperfusion-induced lesions, a direct and serious impact on the patient's prognosis and quality of life. Since 1986, Murry was first proposed after myocardial withstood several brief episodes of ischemia / reperfusion subsequent prolonged ischemia can delay and reduce myocardial injury, and that the concept of ischemic preconditioning, creating a myocardial protection of a new heaven and earth. Due to the occurrence of acute ischemic events is difficult to be very difficult to predict, pretreatment application is greatly limited. In recent years, some scholars have proposed this new method of myocardial protection of ischemic post-processing (Ischemic Postconditioning, I-postC), continued to give several brief reperfusion / ischemia reperfusion myocardial recovery after ischemia. reduce myocardial reperfusion injury, not only with the protective effect of ischemic preconditioning (Ischemic Preconditioning, IPC), and the I-postC after the onset of ischemia, and therefore have a better prospect for clinical application. The experiment is based on validation ischemia after treatment of myocardial protective effects. Objective rat myocardial ischemia / reperfusion model of ischemic treatment of ischemia / reperfusion myocardial protective effects. Research content and methods of health SD rats 30, weight 250 ± 30g, were randomly divided into three groups, sham group (sham operation, sham group), ischemia / reperfusion (Ischemia / - Reperfusion, the I / R), lack of blood post-treatment group (Ischemic Postconditioning, I-postC). Model of to establish myocardial ischemia / reperfusion. Ischemia reperfusion group, tighten the ligature ischemia for 30 min, to relax ligature reperfusion 180 min; treated after ischemia, after 30 min of ischemia and reperfusion 10 s, 10 s of ischemia, three consecutive cycles then reperfusion 179min; collar sham group after thoracotomy threading do, but do not tighten the ligature. Determination of MDA, SOD, CK area of ??content and myocardial ischemia, myocardial necrosis zone area. After ischemia and reperfusion, ischemia-reperfusion group and the treatment group after ischemia serum MDA was significantly higher than the sham group, serum SOD activity was significantly lower than the sham group (P lt; 0. 01), ischemia serum MDA content of the treatment group was significantly lower than the ischemia-reperfusion group, serum SOD activity was significantly higher than the ischemia-reperfusion group (P lt; 0, 01). After reperfusion, the treatment group after ischemia and ischemia-reperfusion group creatine kinase activity was significantly higher than that in the sham surgery group (P lt; 0.01) after ischemia treatment group was significantly lower than ischemia-reperfusion group (P lt; 0.01). Reperfusion after ischemia treatment group and ischemia-reperfusion group of myocardial ischemia and left ventricular area ratio was no significant difference (P gt; 0.05) after ischemic myocardial necrosis area treated with the ischemic area ratio was significantly low in ischemia-reperfusion group (P lt; 0.01). Conclusion ischemic postconditioning have definite myocardial protection function, can improve heart function, reduce oxidative stress injury, relieve ischemia / reperfusion myocardial necrosis. Its mechanism of action: 1, to promote the release of endogenous substances, reducing oxygen free radical oxidative damage; 2, reduce myocardial infarct size, reduce the release of cardiac enzymes.
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