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Objective: aquaporin -4 (Aquaporin 4, AQP4) is the brain of the most widely distributed water channel protein , in the formation of a variety of reasons ( such as trauma , tumor , ischemia ) caused by cerebral edema (brain water content, BE) both play an important role . The present study, the dynamic observation of severe burns juvenile rat brain water content ( brain water content , BWC ) , the content of brain tissue Evans blue (Evan's blue, EB) and brain AQP4 expression Nimodipine (Nimotop) changes explore serious burns brain edema formation mechanism and Nimotop intervention mechanism provided the experimental basis for clinical treatment . Methods : health juvenile one -month-old Sprague-Dawley (SD) rats 72 weight 70 ~~ 100g , male or female, to establish a 30% TBSAIII severely scalded rats model . Were randomly divided into four groups : A ( control group , n = 6) ; B ( sham group , n = 6) ; C ( burn group , n = 30) ; D ( burns Nimotop treatment group , n = 30 ). C, D group was divided 2,6,12,24,48 h 5 time points after severe burns . Wet and dry weight measured BWC; the UV spectrophotometer brain tissue EB content ; the immunohistochemical staining observe brain AQP4 expression . Results: Severe Scald the rat brain BWC and AQP4 expression were increased , 2h began to increase and reached a peak at 6h , 48h still higher ; BWC changes with changes in AQP4 expression was a significant positive correlation ( r = 0.937 , P lt; 0.05). Blood-brain barrier (blood-brain barrier, BBB) permeability ( EB content BBB permeability ) burns after 2h began to increase and peaked at 6h ; BBB permeability and AQP4 expression was a significant positive correlation ( r = 0.967, P lt; 0.05). Application Nimotop after AQP4 expression down , BBB permeability decreased , BWC significantly decreased . Conclusion: Severe burns AQP4 expression in the brain of young rats , BBB permeability and BWC increased expression of AQP4 variation was positively correlated with the BBB permeability and BWC , shows that of AQP4 with serious burns brain edema formation and BBB damage The existence of a significant positive correlation . The AQP4 raised may be one of the reasons that caused the the BBB open and BWC increase . Given Nimotop treatment compared with the burn group at respective time points , the AQP4 expression BBB permeability and BWC significantly differences (P lt; 0.05), table Ming Nimo same may reduce the expression of AQP4 protection BBB the function so as to reduce serious burns cerebral edema .
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