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Purpose of establishing the rabbit high cholesterol - atherosclerosis model , observed in serum lipids , lipoprotein-associated phospholipase A2 (lipoprotein-associated phospholipase A2, LP-PLA2) concentration , and explore the lipoprotein-associated phospholipase A2 and rabbit atherosclerotic ( atherosclerasis As) and simvastatin intervention effect . 56 healthy 6 -month-old male New Zealand white rabbits weighing 2.0 ± 0.2kg, adaptive feeding after a week , were randomly divided into three groups: normal control group and high-fat diet group ( 4 weeks, 8 weeks , 12 weeks ) each time point 8 ; simvastatin group : simvastatin continuously in the high-fat diet for 4 weeks after 8 weeks , each 3mg/kg.d , a total of eight . , Respectively, at 4 weeks, 8 weeks , 12 weekends in the early morning awake by the rabbit ear artery specimens from fasting blood specimens ( fasting for 12 hours ) , centrifuged ( 3000rpm , 10min ) specimens from serum frozen at -20 ℃ . Measured by ELISA in serum total cholesterol ( total cholesterol , TC) , triglycerides ( triglyceride , TG ) , LDL ( low destiny lipoprotein , LDL ) , high density lipoprotein (high destiny lipoprotein, HDL) , and lipoprotein phospholipase A2 (LP-PLA2) concentration; abdominal aorta , thoracic aorta pathological morphology . 1 ) normal control group, blood lipids and LP-PLA2 concentration in 4,8,12 weekend , no statistically significant difference . 2) 4, 8 and 12 weeks after the high - fat diet fed the high fat diet group compared with the control group , the former serum TC, LDL, LP-PLA2 levels than the latter significantly elevated ( p lt; 0.01 ) ; former serum HDL elevated than the latter (p lt; 0.05), but with time , the overall downward trend . 3 ) high-fat diet fed after 8 weeks of high fat diet group compared with the normal control group , elevated serum TG (p lt; 0.01). 4 ) simvastatin group : 8 weeks , compared with the high-fat diet group , serum LP-PLA2 content decreased , but not statistically significant ( p gt; 0.05 ) ; 12 weeks , with the high-fat diet group phase ratio , serum LP-PLA2 decreased (p lt; 0.01). Conclusion 1 LP- PLA2 as a new inflammatory factors involved in the formation of As . 2 . Simvastatin to reduce the role of lipoprotein-associated phospholipase A2 concentration .
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