Dissertation > Excellent graduate degree dissertation topics show

Immune Regulatory Effect of Human Unbilical Cord Mesenchymal Stem Cell on T Lymphocvte of Aplastic Anemia

Author: ChenXin
Tutor: ShiQingZhi
School: Nanchang University
Course: Internal Medicine
Keywords: human unbilical cord mesenchymal stem cells aplastic anemia T lymphocyte immunregulatory effect
CLC: R556.5
Type: Master's thesis
Year: 2009
Downloads: 109
Quote: 0
Read: Download Dissertation

Abstract


Objective: To study the immunomodulatory functions of human unbilical cord mesenchymal stem cells on bone marrow T-lymphocyte of immune mediated aplastic anemia.Methods: MSCs were isolated from normal human unbilical cord,cultured in vitro. T lymphocytes were isolated from bone marrow of immune mediated aplastic anemia by density gradient centrifugation method. The MSCs and T lymphoeytes were co-cultured. The proliferation of lymphocytes was measured by MTT method, the phenotypes of the T cells were analysed by flow cytometry before and after coculture with MSC and the expression of the two marrow hematopoietic factors of aplastic anemia were evaluated by ELISA.Results: (1) In intro,HUC-MSCs were generated successfully. The second generation of MSCs had tyical morphology of fiberblast,and were high expression of CD166,CD29,CD54,low expression of CD13,CD34,CD45 . MSCs could be induced to adipocyte. (2) HUC-MSCs showed inhibitory effect on LCs of aplastic anemia proliferation when HUCMSCs and LCs mixed at the ratio of 1:1、0.5:1、0.1:1, and their inhibitory rate were 56.2±12.1%、43.7±10.4%、28.6±8.9%、3.41±7.6%; HUCMSCs can adjust the proportion of CD4+ and CD8+ T cell subset; and HUCMSCs also can inhibit the secretion of IL-2 and IFN-γby T cells of aplastic anemia.Conclusion:Human unbilical cord mesenchymal stem cells can mediate a immunregulatory effect of T lymphocyte of immune mediated aplastic anemia in vitro and the inhibitory effects are dependent on the amount of HUC-MSCs.

Related Dissertations

  1. Therapeutic Effects of TACI-Ig on Systemic Lupus Erythematosus-like Mouse Model Induced by Active Chromatin Isolated from ConA-actived Lymphocytes,R593.241
  2. Cysteamine on T Lymphocyte Proliferation in Post-Operation Goats and Its Mechanism Underlying,S858.27
  3. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  4. Nutritional composition aplastic anemia mice effect of mitochondrial damage restoration,R556.5
  5. Protective Effect of rhPTH on Mice Hematopietic Injury,R551.3
  6. The Qualitative and Semi-quantitative Analysis of Technetium-99m Labeled Sulfur Colloid Bone Marrow Imaging in the Diagnosis of the Children Aplastic Anemia,R725.5
  7. Clinical Observation on Allogeneic Hemotopoietic Stem Cell Transplantation for Patients with Severe Aplastic Anemia,R556.5
  8. Clinical Efficacy of Cyclosporine a with Stanozolol in the Treatment of 30 Children Patients with Chronic Aplastic Anemia,R725.5
  9. Heterogeneous experimental study of biological bone screw,R687.3
  10. Generation of Mature Dendritic Cells from Peripheral Blood Mononuclear Cells by Calcium Ionophore A23187 and GM-CSF in Vitro,R392
  11. Changes of Seng in the Serum and Umbilical Artery Flow in Pre-Eclampsia,R714.244
  12. The Brain Diffusion Tensor Imaging and Pathogenesis of Cognitive Impairment in Multiple Sclerosis,R744.51
  13. The Study of the Relation among the Concentration of FK506, the Level of T Cell Subsets and Peripheral Blood Mononuclear Cell HBV DNA after Liver Transplantation,R657.3
  14. Study on Correlation of Cellular Immune and Intestinal Permeability After Severe Trauma-hemorrhagic Shock in Rats,R605.971
  15. Study on the Acting Mechanism of Qing Wei Li Chang Capsule in Rats with Experimental Ulcerative Colitis (UC),R285
  16. The Effect of GuBenKeLi on the Lymphocyte and the Contents of Cytokines in COPD Rats Model,R285.5
  17. TWP intractable Graves disease clinical research,R581.1
  18. The Clinical Significance of T Cell Subgroups and NK Cell’s Expression Level in Diffuse Large B-cell Lymphoma Patients,R733.1
  19. The Correlation of T Lymphocytes Subsets and Costimulators in Patients with Type 2 Diabetes with or without Diabetic Nephopathy,R587.2
  20. Patients with alopecia areata T lymphocyte subsets and pathological significance,R758.7
  21. Bioinformatics Analysis of Leucine-rich Repeat Motifs Molecular and VLRs in the Lamprey,Q75

CLC: > Medicine, health > Internal Medicine > Blood and lymphatic system diseases > Blood diseases > Anemia > Aplastic anemia and bone marrow sclerosis,anemia
© 2012 www.DissertationTopic.Net  Mobile