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In recent years, miniature pigs are increasingly being used in medical animal models, transgenic animals, cloning, nuclear transplantation, organ transplant, embryo transfer and embryo implantation in the state-of-the-art research process, and reasonable anesthesia has become these research projects must be resolved problem. Small pig the anesthesia preparation-XFM is developed based on the theory of balanced anesthesia and physiological characteristics of the miniature pig. The purpose of this project is to study XFM small blood pressure, heart rate and blood related cytokines, neuropeptides and neurotransmitters, so as to explore the effect and mechanism of XFM miniature pigs hemodynamics. This test is based on the Chinese experimental miniature pigs for experimental animals by intramuscular injection miniature pigs composite anesthetic-XFM its serum nitric oxide (NO), plasma endothelin (ET), the forefront of prostacyclin (PGI2) and thromboxane A2 (TXA2), renin - angiotensin - aldosterone (RAAS), atrial natriuretic peptide (ANP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and nerve neurotensin (NT), etc. The concentration of dynamic monitoring of XFM miniature pigs hemodynamic effect and mechanism. 14 Chinese experimental miniature pig intramuscular XFM 0.15 mL kg-1, prior to the injection after injection 5,10,30,45,60,80 and 120 min, respectively, systolic blood pressure (SBP), diastolic blood pressure (DBP ), mean arterial pressure (MAP) and heart rate (HR) monitoring and synchronization take the front chamber of venous blood samples. Blood samples were immediately placed in test tubes containing different anticoagulants and inhibitors to the corresponding pre-treatment, and using the colorimetric method and radioimmunoassay were determined. The results showed that miniature pigs after intramuscular injection of 0.15 ml kg-1 of XFM, showed good anesthesia: rapid induction of anesthesia, anesthesia was maintained appropriate time, steady wake; analgesic, sedative and muscle relaxant results are satisfactory and balanced. Small and medium-sized pig during the test showed significant hemodynamic changes, as demonstrated by the SBP, DBP, MAP and HR after injection showed increased at first, and then decreasing trend, but changes in these indicators in miniature pigs within a physiologically tolerable range. Miniature pigs after the injection XFM its hemodynamic changes result with serum NO, plasma ET, 6 - keto - F1α (6-keto-PGF1α) and thromboxane B2 (TXB2) content for multivariate two the two correlation analysis showed that plasma ET, 6-keto-PGF1 and TXB2 concentration and blood pressure changes have a significant or very significant, and no correlation between changes in NO and hemodynamic parameters, indicating that plasma ET, 6-keto -PGF1 and TXB2 to participate in the regulation of the XFM miniature pigs hemodynamic changes. Miniature pigs after the injection XFM, plasma renin activity (PRA), angiotensin II (A II) and aldosterone (ALD) concentration and SBP, DBP, MAP and HR showing a similar trend, and between them a certain degree of correlation. Thus, PRA, A Ⅱ and ALD involved the XFM miniature pigs caused by hemodynamic changes in the process, RAAS change may be one of the mechanisms is the XFM caused hemodynamic changes. Miniature pigs after the injection XFM, the hemodynamic changes in the results of plasma in NPY, NT, CGRP and ANP concentration multivariate pairwise correlation analysis showed that: ANP was negatively correlated with SBP and MAP, and DBP was significantly negatively related; CGRP and SBP, DBP, and MAP was a negative correlation; NPY and NT is no correlation with the changes of the hemodynamic parameters. The results indicate that CGRP in plasma ANP concentration is caused XFM after injection small pig hemodynamic indicators change is an important factor. This study shows that: the small pig injection XFM 0.15 mL kg-1 showed significant hemodynamic changes and hemodynamic changes in the indicators and plasma ET, 6-keto-PGF1, TXB2, RAAS, CGRP and ANP concentration changes were significant or very significant correlation. Therefore, after injection of XFM plasma ET, 6-keto-PGF1, TXB2, RAAS, CGRP and ANP concentration changes may be one of the main reasons leading to the small pig hemodynamic parameters change.
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