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The Study of Isolation and Purification by Ultra-filtration of Duck Embryo Peptide and Its Functional Properties

Author: WangXuQing
Tutor: ZhangPeiZheng；LiDaPeng
School: Shandong Agricultural University
Course: Of Food Science
Keywords: Duck Peidan polypeptide Ultrafiltration Purification Antioxidant Anti-fatigue Hypoxia tolerance
CLC: R151
Type: Master's thesis
Year: 2009
Downloads: 156
Quote: 1
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Abstract


Duck the embryo egg is fertilized duck eggs with a special hatching process the embryo continuous development, with the vitality of the duck, its high protein content, and contains some of the ingredients of the human placenta, improve digestion, anti-oxidation, anti-aging effect; addition during growth and development of the embryo may produce large amounts of nutrients, such as peptides, small molecular weight peptides with molecular weight proteins can be better compared to more quickly absorbed by the body, so you can use duck Peidan to development health food market prospects are very bright. The test raw materials duck Peidan as the ultrafiltration separation and extraction duck Peidan polypeptide, to determine a suitable ultrafiltration conditions; dextran gel Sephadex-G25 column chromatography purification the duck Peidan polypeptide; effect of different molecular weight segment the duck Peidan polypeptide solubility, foaming and sparkling stability, emulsification and emulsion stability, in vitro antioxidant in vivo antioxidant, anti-anoxia, anti-fatigue effect of such features. The main results are as follows: 1. Extract by ultrafiltration the Cherry Valley SM3 the Duck Peidan polypeptide when the shut-off ultrafiltration membrane having a molecular weight of 50000Da should be at an operating pressure of 0.08 ~ 0.1Mpa, the operating temperature of 35 ° C, the feed liquid itself running under the pH conditions 30min for a duty cycle; MWCO ultrafiltration membrane 13000Da operating pressure 0.08 ~ 0.1Mpa Operating Temperature lt; 45 ° C, solid to liquid pH conditions run for 30 minutes to a cycle; closure molecular weight of 10000Da the ultra-filtration membrane operating pressure 0.08 ~ 0.1Mpa Operating temperature LT; 45 ° C, solid to liquid pH conditions 40min for a duty cycle; 6000 Da molecular weight cutoff ultrafiltration membrane operating pressure 0.08 ~ 0.1Mpa Operating temperature LT; 45 ℃, solid to liquid pH conditions and run for 60 minutes for a duty cycle; shut-off ultrafiltration membrane with a molecular weight of 3000Da operating pressure 0.08 ~ 0.1Mpa Operating Temperature lt; 45 ° C, solid to liquid pH conditions and run for 60 minutes a duty cycle. Ultrafiltration extraction the Ma Duck Peidan of polypeptide, the shut-off molecular weight the ultrafiltration membrane 50000Da should at an operating pressure of 0.08 to 0.1Mpa Operating temperature 35 ° C, the solid to liquid pH conditions 20min for a duty cycle; MWCO for ultrafiltration membrane of 13000Da should the operating pressure of 0.08 to 0.1Mpa Operating Temperature lt; 45 ° C, the material liquid pH conditions 40min for a cycle; closure molecular weight of 10000Da the ultra-filtration membrane at a pressure of 0.08 to 0.1 Mpa Operating temperature lt; 45 ° C, the solid to liquid pH conditions run 40min for a duty cycle; molecular weight cutoff of 6000 Da ultrafiltration membrane at an operating pressure 0.08 ~ 0.1Mpa Operating Temperature lt; 45 ° C, the solid to liquid pH conditions run for 60 minutes a cycle; closure molecular weight of 3000Da ultrafiltration membrane should be operating pressure of 0.08 to 0.1Mpa Operating temperature LT; 45 ° C, solid to liquid pH conditions and run for 60 minutes a cycle. 3 dextran gel Sephadex-G 25 column chromatography polypeptides of different molecular weight segment of Ap Peidan of ultra-filtrate was further purified, and the physicochemical properties of analysis, the results show two cultivars Ap Peidan of polypeptide have better solubility, foaming and foaming stability, emulsifying and emulsion stability, in vitro antioxidant activity shown by animal testing, two species of duck embryonated eggs polypeptide can affect mice antioxidant, anti- fatigue, hypoxia tolerance.

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CLC: > Medicine, health > Preventive Medicine,Health > Nutrition, hygiene,food hygiene > Nutrition
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