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Purpose Vincent BH et al found that C57BL / 6 mice of the pituitary, thyroid, bone marrow tissue, the expression a TSHβ splice variants, its transcription initiation in intron 4 of the 3 'end 27 bases, so it is missing the first 4 exon containing the entire coding region of exon 5. Followed by Jeremy S reported human pituitary TSHβ splice variants also expressed in the peripheral blood and thyroid. TSHβ splice variant and mice are very similar, containing intron 2 of the entire coding region of 27 nucleotides and exon 3. It is the first time been found TSHβ contains part of intron splicing variant, preliminarily confirmed TSHβ splice variant of the mouse and in vitro experiments with biological activity. But, so far, for it is part of the expression, the physiological and pathological significance is unclear. We confirm the the TSHβ splice variants expressed in Balb / c mice pituitary, thyroid, bone marrow, spleen, peripheral blood and lymph case, the use of the LPS preparation mice endotoxin-induced systemic inflammatory response syndrome (systemic inflammatory response syndrome SIRS) model, through detection TSHβ splice variant expression changes in the pituitary, thyroid, bone marrow, spleen, and explore the role of the body from infection TSHβ splice variants of the levels of thyroid hormones in the regulation loop. Methods In this study, first select the 36 male and female of Balb / c mice, 7-8 weeks old, were randomly divided into 6 groups (n = 6), respectively: 0h, 6h group, 12h, 24h group, 48h group, 72h group. 0h group: intraperitoneal injection of saline; Other groups were confirmed by intraperitoneal injection of LPS, 3mg/kg body weight. Group according to the set time, take the whole blood of the animal, after injection using RIA and ELISA were used to detect serum thyroid hormone and thyroid-stimulating hormone levels. Using the PCR technique to detect the TSHβ splice variant expression in Balb / c mice pituitary, thyroid, bone marrow, spleen, peripheral blood, lymph nodes, and thyroid the amplification product of the gene were sequenced. SYRR GREEN real-time fluorescence quantitative PCR analysis the TSHβ splice variants under different processing time gene mRNA expression. Results 0h serum TT4 level of 24.5875 nmol / L, compared to the other groups were decreased (P lt; 0.01) for 24 h decreased the most obvious 9.3283 nmol / L, 48 h, starting until 72h 18.696 nmol / L still not return to normal levels. TSH levels did not change significantly. PCR results showed that the the TSHβ splice variants expressed in the pituitary, thyroid, bone marrow, spleen, peripheral blood, lymph nodes, but apart from the other organizations outside the pituitary do not express the natural type the thyrotropin β. TSHβ splice variant expression in the bone marrow, 6h, 12h, 24h decreased compared to 0h, 48h back to normal, 0h (1.000 ± 0.000) and 6 h (.511027 ± 0.10390) (p lt; 0.01) and 12 h (0.36788 ± .06024) (p lt; 0.01), 24 h (0.198357 ± 0.03266) (p lt; 0.01). Thyroid TSHβ splice variant expressed in 6h 0h increased, 0h (1.000 ± 0.000), 6h (10.43319 ± 4.86834) (P lt; 0.01). The other point in time than 0h compare the thyroid TSHβ splice variant expression were not statistically significant. The TSHβ splice variants and natural TSHβ expression at each time point were no significant changes in the pituitary gland. The conclusion 1.PCR results show Balb / c mice pituitary, thyroid, bone marrow, spleen, peripheral blood, lymph node tissue expression TSHβ splice variant the natural type the TSHβ only in pituitary expression. 2 intraperitoneal injection of LPS (3mg/kg body weight) can reduce circulating levels of thyroid hormones on TSH had no significant effect. 3.LPS make the TSHβ splice variants mRNA expression in Balb / c mice thyroid tissue significantly increased, but LPS suppression of bone marrow TSHβ splice variant mRNA expression prompted the TSHβ splice variants involved in inflammation reaction in the immune - endocrine network The regulator has an important role in the physiological and pathological role and regulation of the immune system sources TSHβ splice variants mechanism needs further research.
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