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Objective: To study the role of palmitic acid in 3T3-L1 adipocytes cells affect AQP7 in palmitic acid signaling pathway affect AQP7 expression explore . Methods: 3T3-L1 preadipocytes cultured in vitro study , induced to differentiate into mature fat cells , oil red O staining of cell differentiation and maturation rate . Palmitic acid palmitic acid role in mature fat cells , different concentrations of the enzyme colorimetric assay for 24h adipocyte glucose utilization and glycerol release changes . RT-PCR to observe the impact of fat cells AQP7 mRNA, PPARγmRNA expression levels of palmitic acid effect of different concentrations and different time . Results : 3T3-L1 preadipocytes during induction , cell morphology becomes large , an increase in intracellular lipid droplets ; the effect of palmitic acid , with the increase of the concentration of palmitic acid role , the cells on the glucose in the medium by gradual reduction of each group compared with control group , has a significant difference ( P LT ; 0.01 ) ; fat cells release of glycerol in the medium was gradually decreased , and each group is compared with control group , has a significant difference ( P LT ; 0.01 ) ; 100 μM , 200μM , 400μM palmitic acid concentration group compared with the control group , the fat cells AQP7mRNA expression levels were down 16% , 30 % , 41 % , PPARγ mRNA expression levels were decreased by 10% , 22% , 31% , and the difference was statistically significant ( P lt; 0.05 ) ; 200μM palmitic acid treatment fat cells 12 h for 24 h after 48 hours , conducted between-group comparison , 12h and 48h , 24h group the 48h group the fat cells AQP7mRNA expression level between the difference was statistically significant (P lt; 0.05), 12h group with the 48h group the fat cells PPARγmRNA expression level between the difference was statistically significant ( P lt; 0.05 ) . Conclusion: palmitic acid may affect fat cells AQP7 expression , thus contributing to the insulin resistance of fat cells . Palmitic acid by PPARγ nuclear receptors may in turn affect the AQP7 expression of the fat cells .
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