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TGF-β1 in rats with alcoholic liver disease expression meaning

Author: CaiJieChao
Tutor: TanYunBo
School: Dali University
Course: Surgery
Keywords: Liver , alcoholic TGF-β1
CLC: R575.2
Type: Master's thesis
Year: 2011
Downloads: 33
Quote: 0
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Abstract

Objective: To observe the TGF-β1 (transforming growth factor β1) in alcoholic liver disease and liver serum levels to explore TGF-β1 in alcoholic liver fibrosis. Methods: 55 male Wistar rats (weighing 200-250 g), normal feeding a week later, were randomly divided into control and experimental groups: (1) Daily 10:00 experimental group with 56% ethanol [8g / (kg.d ) gavage once, for 12 weeks: control rats with normal saline irrigation and other capacity; at a temperature of 20 ~ 26 ℃, humidity (70% -75%) of clean grade Dali University animal laboratory housed separately normal diet, free access to water. (2) the experimental group were sacrificed on the first weekend 4,8,12 10 rats, the test steps are: rats were fasted for 12h and weighed, with 10% chloral hydrate (0.3ml/100g) anesthetized by intraperitoneal injection, blood through the femoral artery, and then separation of serum, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and TGF-β1 levels. Disinfection of the rat liver after laparotomy excised liver weight were weighed, and in accordance with (liver index = liver weight / body weight × 100%) of the liver index calculated. (3) fixed with 10% formaldehyde solution in liver tissue, paraffin cuts, HE staining, observed under the microscope with alcoholic liver disease in rats at different stages of liver morphological changes; immunohistochemical ABC method TGF liver tissue -β1 content in different periods were detected by ELISA in serum TGF-β1 concentrations. (4) using SPSS16.0 statistical software for analysis. TGF-β1, ALT, AST results with x ± s said that each group using T test. P lt; 0.05 as statistically significant difference. Results: (1) Section 8 and 12 weeks of liver tissue of experimental rats with chronic alcoholic liver disease in all its manifestations. Week 4: liver cell edema and disarranged, local ballooning degeneration; 8 weeks: uneven periportal lymphocytic infiltration; 12 weeks: periportal great expansion of central venous congestion, local mild fibrosis. (2) between the experimental group at each time point of serum ALT and AST were significantly (P lt; 0.05), ALT and AST each time point the experimental group and the control group there was significant difference between (P lt; 0.01 ). (3) experimental serum Section 8 and 12 weeks and the control group TGF-β1 levels (ng / ml) by analysis of variance at each time point TGF-β1 was significant difference between (P lt; 0.05), the first 8 and 12 weeks compared with the control group, there was significant difference between (P lt; 0.05). (4) TGF-β1 immunohistochemistry results showed that the control group, TGF-β1 was weakly positive in the experimental group showed brown TGF-β1 expression, mainly in the sinus wall, the central vein and portal area around the adjacent liver expressed as the amount of cytoplasm aggravation of liver damage increases, TGF-β1 expression levels strengthened.

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