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Objective: To investigate panax notoginseng (panax notoginseng saponins, PNS) pretreatment protective effect of autologous liver transplantation ischemia-reperfusion injury in rat partial liver resection , observation of TNF-α, IL-1, HIF-1α, Caspase 3 protein expression and apoptosis . Methods: The rat partial hepatectomy autologous liver transplantation animal model . The pure male SD rats of 54 , based on different pretreatment drugs before reperfusion , PNS preconditioning group (PanaxNotoginseng Saponins group, PNS group) were randomly divided into saline control group (Control group, CM group) and sham-operated model (sham operation, SO group ) , 18 in each group . Rats respectively autologous liver transplantation reperfusion or sham surgery group after 2h , 6h , 24h at each time point , animals in each group were sacrificed , serum alanine aminotransferase ( ALT ) , aspartate aminotransferase (AST) levels ; HE stained sections do histopathological examination , TUNEL assay of apoptosis ; immunohistochemical detection of TNF - α, IL -1 , HIF -1α , and Caspase -3 protein expression . Results : (1) CM group and PNS group in 2h , 6h and 24h at each time point of serum ALT and AST levels and apoptotic index were significantly higher than S0 group ( P <0.01 ) ; ( 2 ) 2h, 6h and 24h each point in time , CM serum ALT and AST levels , apoptotic index , TNF -α , IL -1 , caspase -3 protein expression was significantly higher than the PNS group ( P <0.01 ) ; ( 3 ) in 2h, 6h and 24h each point in time , the PNS group HIF-1α protein expression was significantly higher than the CM group ( P <0.01 ) . Conclusions : ( 1 ) rat partial hepatectomy autologous more serious liver after liver transplantation ischemia-reperfusion injury , its mechanism involving oxygen free radicals increases, changes in cytokine levels and apoptosis . (2) PNS pretreatment reduces rat autologous hepatic ischemia- reperfusion injury after liver transplantation , which may be related to inhibition of apoptosis . (3) PNS promote by inhibiting apoptotic gene caspase -3 , TNF - α expression inhibits apoptosis . (4) HIF-1α can reduce the inflammatory cytokines of TNF- α and IL-1 levels, thereby reducing ischemia-reperfusion injury .
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