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Research on the Effects and Mechanism of Molluscacidal with Rongbao

Author: LiGuangPing
Tutor: HuPingCheng;ZhaoZhengYuan
School: Central South University
Course: Public Health
Keywords: Rongbao Calcium cyanamide Snails Molluscicide Mechanism
CLC: R184
Type: Master's thesis
Year: 2009
Downloads: 30
Quote: 1
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Abstract


Objective: To evaluate the effect of Rongbao molluscicidal explore suitable scene of the spraying dose and method; the the Rongbao effectively kill snails ingredients its MOLLUSCICIDES effect; explore Rongbao role effectively kill snails component induced histological changes in snails to study the the Rong Bao molluscicide mechanism to provide a scientific basis for the comprehensive scientific popularization and application. Methods: kill and sprayed kill method of soaking in the laboratory and field Rong Bao MOLLUSCICIDAL test, while using mechanical power dusting method in the field molluscicidal test, the molluscicidal effect of the drug was observed; soaking mode centrifugation the Rongbao the leaching solution by killing snails test, and test, comparison test, to explore effective Rongbao MOLLUSCICIDES ingredients; and through continuous pathological analysis of histological changes Rongbao effective to kill snails ingredients snail. Results: Rong Bao 50mg / L and 80mg / L dose Baptist kill 5d, indoor snails mortality rate of 70% and 100%, respectively, site natural ditches within the snail mortality of 81.29% and 94.59%, respectively. Rongbao 30g / m to 2 dose spray 1d, indoor snails mortality rate was 100%. Scene pesticide spraying and dusting method 7d snails mortality of 80.38% and 70.73%, respectively; Rong Bao, 25, 50, 100, 200 mg / the L dose 24h 72h of snails lixivium mortality rates were 20%, 85%, 100 % and 100% Rong Bao 200mg / L dose of 1-5 days of continuous leaching solution dip kill 48h, snails mortality rates were 100%, 10%, 5%, 5%, 15%. Rongbao lixivium 200nm, 205nm and 215nm wavelength absorption peak. At 205nm wavelength, the Rong Bao 25, 50, 100 mg / L dose of fluid and Cyanamide 6.25,12.5,25 mg / L dose solution 0D were 0.021,0.070,0.172,0.028,0.072 and 0.171. 48h Baptist kill the snails mortality were 40%, 95%, 100%, 20%, 90% and 100%; snails tissue sections mantle epithelial cells to crack, flake after the Rongbao 200mg / L dose lixivium treatment, basal layer bare, reduced secretory granules. The branchiostegal cell expansion, the surface was a burr-like central vascular rupture. The smooth muscle cells of the gastropod edema expansion, cell membrane fusion, was hyalinization. The hepatic lobule shrinking intrahepatic vascular expansion rupture, a significant reduction in the secretory granules. Conclusion the: Rong Bao 50mg / L dose Baptist killing dose of 30g / m ~ 2 Pensha has a the strong MOLLUSCICIDES effect. Dusting molluscicide effect of site Rongbao better than spraying, you can select the method of application in the light of local conditions; Rong Bao is slightly soluble substances, to kill snails effective ingredients in the short term by hydrolysis to release most of, sediment the Rongbao after the separation of the leaching solution. No kill Lo effect Rongbao do not have a release molluscicide features. Rong Bao is a kind of mixture, the main ingredient is calcium cyanamide. Spectrophotometry verifiable leaching solution containing Cyanamide, effectively kill the snails ingredients leaching solution containing other impact MOLLUSCICIDES ingredients needs further study; Rongbao leaching solution under the mantle of the snails, branchiostegal gastropods and liver have significant pathological changes. Especially respiratory and liver damage is a direct result of the death of the snails.

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CLC: > Medicine, health > Preventive Medicine,Health > Epidemiology and Prevention of Disease > Prevention measures and management
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