Dissertation > Excellent graduate degree dissertation topics show

A Preliminary Study of Myocardial Protective Role of Nucleolin Using Transgenic Mice

Author: ZhouBin
Tutor: XiaoXianZhong
School: Central South University
Course: Pathology and Pathophysiology
Keywords: nucleolin transgenic mouse myocardial ischemia-reperfusion injury
CLC: R363
Type: Master's thesis
Year: 2011
Downloads: 22
Quote: 0
Read: Download Dissertation

Abstract


【Objective】This study was attempted to investigate the protective role of nucleolin against myocardial ischemia-reperfusion (I/R) injury in nucleolin transgenic mice.【Method】An eukaryotic expression plasmid of nucleolin (pcDNA3.1-Ncl) and a myocardium-specific expression plasmid of nucleolin (Alpha-MyHc clone 26-Ncl) were constructed. Alpha-MyHc clone 26-Ncl plasmid was used to create nucleolin transgenic mice. The genetype of transgenic mice was identified by PCR. The amount of nucleolin gene was evaluated by real-time PCR and the nucleolin protein expression level was tested by Western-bolt. The myocardial morphology, heart weight index (HWI) and left ventricular pressure maximum rise rate (+LV Max dP/dt) were observed in nucleolin transgenic and wild type mice. A myocardial ischemia-reperfusion (I/R) injury model was prepared by ligation of left anterior descending branch (LAD) of coronary artery for 30 min then release for 2hours. Myocardial caspase-3 activity, serum creatine kinase activity and left ventricular pressure maximum rise rate (+LV Max dP/dt) were observed.[Results] pcDNA3.1-Ncl plasmid was constructed with the ability to express nucleolin in myocardial cells. Alpha-MyHc clone 26-Ncl vector was also constructed and transgenic mice which specially express nucleolin in myocardium was prepared. The myocardial morphology, HWI and left ventricular pressure maximum rise rate in nucleolin transgenic mice were similar with those in wild type mice. After myocardial I/R injury, nucleolin transgenic mice showed less caspase-3 activity, less serum creatine kinase activity and higher left ventricular pressure maximum rise rate (+LV Max dP/dt) than those in wild type mice. [Conclusion] Nucleolin protects myocardium against ischemia-reperfusion (I/R) injury in mice.

Related Dissertations

  1. Kangxian Ruangan Granule on Rat Hepatic Stellate Nucleolar Non-histone Protein,R285.5
  2. Sal B on isolated working heart protective effect,R285.5
  3. Experimental Study of the Protective Effect of Pharmacological Precondition Induced by GSTT on Isolated Rat Myocardium,R285.5
  4. Functional Delineation of Azu-1 and PinX1 in Mitosis,Q343
  5. Regulation of the active ingredients of traditional Chinese medicine , applied basic research in the filters on cardiac function of isolated working heart technology,R284
  6. The Effect of Gross Saponins from Tribulus Terrestris L.on Isolated Rat Hearts,R285
  7. Expression and Function of WD-40 Repeat Proteins WDR26 in Myocardial Ischemic Postconditioning,R541
  8. Based on the \,R259
  9. Endothelial Cell-Specific Expression of Cre Recombinase in Transgenic Mice,Q789
  10. Experimental Study on the Recombinant pcDNA3-β-NGF Transfecting BMSCs of GFP Transgenic Mice,R346
  11. The Protective Effect of Shengmai Injection on Myocardium of Rabbit with Ischemia Reperfusion Injury,R285.5
  12. Effects of Rosiglitazone and Fenofibrate on Cardiomyocyte Apoptosis and Expression of Related Proteins after Myocardial Ischemia-reperfusion in Rats,R96
  13. The Miho heart on myocardial ischemia - reperfusion injury in experimental study,R285.5
  14. Protective Effects of Quaternary Ammonium Salt Derivative of Haloperidol （F2） on Myocardial Ischemia-Reperfusion Injury,R96
  15. Neiguan in rabbits with acute myocardial ischemia and reperfusion injury protective effect of delayed,R245.97
  16. Heart Care Po prevention of myocardial preconditioning ischemia-reperfusion injury in clinical and experimental research,R259.4
  17. Dynamic Change of Nitric Oxide Synthetase(NOS)and Its Gene Expression During Myocardial Ischemia and Reperfusion,R654.2
  18. Moxa cone moxibustion tanzhong, Geshu pretreatment on myocardial ischemia - reperfusion injury in cardiac myocyte apoptosis genes influence,R245
  19. A Proteomic Study of S-nitrosylation in the Rat Heart,R341
  20. The Cardioprotective Effect of Qidan Tongmai Tablet Against Myocardial Ischemia/reperfusion Injury and Its Mechanism,R285.5
  21. A Study to the Protective Effects of Tianxiangdan Pellets in Myocardial Ischemia-reperfusion Injury,R285.5

CLC: > Medicine, health > Basic Medical > Pathology > Pathophysiology
© 2012 www.DissertationTopic.Net  Mobile