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Author: DuZuo
Tutor: YuZuo
School: Liaoning University of Traditional Chinese Medicine
Course: Traditional Chinese Medicine
Keywords: Myocardial ischemia-reperfusion injury Ginaton apoptosis Collateral
CLC: R259
Type: Master's thesis
Year: 2011
Downloads: 37
Quote: 0
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Abstract


Purpose:The term experimental study is based on the "network theory of governance" in order to contact the disease theory of medicine based on Chinese medicine by promoting blood circulation to improve the network myocardial ischemia-reperfusion injury in rabbits with myocardial infarct size and no-reflow area, and through the enzyme, cells Apoptosis and expression of gene Ginaton preliminary study on myocardial ischemia reperfusion injury mechanism of myocardial protection, as well as the treatment of collateral disease theory to provide a scientific basis.Material and method:The 50 New Zealand white rabbits were randomly divided into 5 groups, n = 10, sham group, respectively①,②model group,③Ginaton A group 50mg/kg,④Ginaton 100mg/kg and B group⑤nitroglycerin group. Left ventricular anterior descending artery ligation followed by reperfusion 90min 120min, produced myocardial ischemia reperfusion injury model.①sham group completed all experimental operation, wear-lane artery ligation;②model group, intravenous infusion of normal saline by 0.3ml/kg/min;③④group by group and by 0.3ml/kg/min the dose intravenous drip Ginaton;⑤group by 0.1ml/kg/min intravenous nitroglycerin infusion stopped after 120min. After the end of the experiment blood from the jugular vein 5ml cTnT and hs-CRP levels detected; the left ventricular slice area of myocardial no-reflow and myocardial infarct size; will not stain cardiac tissue observed under light microscope after treatment apoptosis index; will the right atrial appendage of the organization by reverse transcription polymerase chain reaction (RT-PCR) methods of myocardial tissue cPLA2-γ-γgene expression levels.Results:1. Ginaton A Group B Group Ginaton and nitroglycerin compared with model group, NRA / LVA and the MIA / LVA was significantly reduced (P <0.01); nitroglycerin group and Ginaton group A compared with group B (P> 0.05), the difference was not statistically significant; Ginaton group B compared with A group, the difference was not statistically significant (P> 0.05).2. Model group and sham group compared, cTnT and hs-CRP levels were significantly higher (P <0.01), Ginaton A group, B group and the nitroglycerin group and model group, cTnT and hs-CRP levels decreased significantly (P < 0.01); nitroglycerin group and Ginaton group A compared with group B (P> 0.05), the difference was not statistically significant; Ginaton group B compared with A group, the difference was not statistically significant (P> 0.05).3. The model group compared with the sham group, the number of apoptotic cells was significantly higher (P <0.01), Ginaton A group, B group and the nitroglycerin group and model group, the number of apoptotic cells was significantly lower (P < 0.01); nitroglycerin group and Ginaton group A compared with group B (P> 0.05), the difference was not statistically significant; Ginaton group B compared with A group, the difference was not statistically significant (P> 0.05)4. Model group, Ginaton group and the nitroglycerin group of cPLA2-γmRNA expression was significantly increased (P <0.01), Ginaton A Group B Group Ginaton cPLA2-γmRNA expression increased in the normal group, but compared with the model group, significantly lower than model group (P <0.01). Nitroglycerin group of cPLA2-γmRNA was higher than the normal group, but compared with the model group was significantly lower than model group (P <0.01). Kim Nadeau A, Ginaton nitroglycerin group B group and no significant difference (P> 0.05).Conclusion:1. Ginaton can significantly reduce the myocardial ischemia reperfusion injury of myocardial no-reflow area and myocardial infarct size, which can reduce the extent of myocardial injury; by Ginaton intervention can reduce the content of cTnT and hs-CRP , said Ming Jinna more myocardial ischemia and reperfusion can reduce the extent of damage, thus helping protect the myocardium. 2. Ginaton can inhibit the myocardial ischemia-reperfusion injury caused by the number of cardiomyocyte apoptosis, reduce the apoptotic index decreased cPLA2-γ-γgene expression, suggesting that Ginaton inhibit cardiomyocyte apoptosis, to effectively protect the myocardium , and its mechanism of action may cPLA2-γ-γgene expression.3. Ginaton two doses of the difference between myocardial protection was not obvious, and nitroglycerin groups comparative results are not statistically significant.

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