Dissertation > Excellent graduate degree dissertation topics show

Human Bone Marrow Mesenchymal Stem Cells Differentiated into Dopaminergic Neuron-like Cells in Vitro.

Author: JinHui
Tutor: ZhangLei
School: Liaoning Medical
Course: Biochemistry and Molecular Biology
Keywords: Human bone marrow mesenchymal stem cells Induce and differentiate neurou-like cells dopaminergic neuron-like cells
CLC: R329
Type: Master's thesis
Year: 2011
Downloads: 18
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveTo explore the potential of human bone marrow mesenchymal stem cells differentiated into neuron-like Cells and dopaminergic neuron-like cells in vitro.MethodsThe human BMSCs were isolated by density gradient centrifugation, and purified by plastic-adhering culture in vitro. Then the purity of BMSCs was analyzed by flow cytometry by detecting CD29、CD44、CD166、CD14、CD34 and CD45. After pretreatment with basic fibroblast growth factor(bFGF) and epidermal growth factor(EGF)48h,then the third passage of BMSCs were cultured at the medium containing glial cell line—derived neurotrophic factor(GDNF) and Angiotensin II(AngⅡ) . The cell morphology of BMSCs during the differentiation was observed by inverted microscope. The expression of Nestin and NSE and TH of BMSCs were detected by RT-PCR in induced group and control group. The expression of NSE and Nestin and TH during the induced group and the control group was detected by IF cytochemistry.ResultsThe third passages BMSCs were homogeneous fusiform shape under the light microscope, the high purity of BMSCs were collected and indicated to express CD29、CD44 and CD166,but not CD34 and CD45. The results showed that 2 weeks after the differentiation of BMSCs by AngⅡthe majority of the BMSCs were transformed into neuron-like cells. There were several processes (axon-like or dendrite-like) from the cell body. The Nestin and NSE mRNAs expressed by BMSCs after induction were more than those before induction significantly (P < 0.05). The result of IF cytochemistry indicate that the expression of NSE、TH increased significantly after induction (P < 0.05), but the GFAP was nagtive whatever before or after induction.ConclusionsThe high purity of BMSCs were gain through the density gradient centrifugation and plastic-adhering culture in vitro, the expression percentage of CD29、CD166and CD44 were above 95%. but no CD34 and CD45. AngⅡcan induce Human BMSCs to differentiate into neuron-like cells in vitro, the neuron-like cells express Nestin and NSE、TH,but not GFAP, Above of all , BMSCs can become the resource of transplantation in neurogenic disease.

Related Dissertations

  1. Four-point bending stress stimulation of human bone marrow mesenchymal stem cell proliferation activity,R329
  2. The Effects of Liraglutide on Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Insulin-producing Cells,R587.1
  3. Bone Marrow Derived Mesenchymal Stem Cells Transplantation Enhances VEGF Expression and Angiogenesis in Myocardium after Myocardial Infarction,R542.22
  4. Biological Characteristics in Vitro of Bone Marrow Derived Mesenchymal Stem Cells from Different Resources and the Use of Human Bone Marrow Derived Mesenchymal Stem Cells in Hematological Stem Cell Transplantation,R329
  5. Biological Responses of Osteoblast-like Cells in Three Dimensional Culture under Cyclic Mechanical Stretch and Its Mechanism,R329.2
  6. Genome-wide Analysis of Histone H3 Lysine9 Modifications in Human Mesenchymal Stem Cell Osteogenic Differentiation,Q343
  7. Effect of Slingshot-1L Phosphatase on Cardiomyocytes Differentiation of Human Bone Mesenchymal Stem Cells,R329
  8. Human bone marrow stem cell characteristics and electro-acupuncture induced osteogenic differentiation of experimental study,R245
  9. Experimental Study of Tissue-engineered Bone Constructed by Osteoinduction Active Material with Hunman Bone Mesenchymal Stem Cells,R318.08
  10. Experimental Study of Transplantation of Human Mesenchymal Stem Cells in Treatment of Experimental Allergic Encephalomyelitis in Rats,R744
  11. Expression of Cell Surface Oligosaccharides LeX/Y and FUT4 is Increased during the Neural Differentiation of Human Bone Marrow Mesenchymal Stem Cells in Vitro,Q813
  12. The Empirical Study of the Animal Model of the RatBone Marrow Stem Cells’s Culture, Introduced into Muscle Skeletal Cells for Treatment Muscle Skeletal Injury,R687.2
  13. The Experiment of Induce and Differentiation Mesenchymal Stem Cells into Bone Cells and Building Tissue Engineering’s Bone Tissue,R318
  14. Human Mesenchymal Stem Cells Differentiate into Epidermal-like Cells in Vitro,R329.2
  15. Preparation of Monoclonal Antibodies Against Human Mesenchymal Stem Cells(hMSCs) and Its Analysis of Biological Characteristics,R392
  16. Human bone marrow mesenchymal stem cell transplantation in the rat brain,R651
  17. Clinical Observation 5 - azacytidine in bone marrow mesenchymal stem cells differentiate into cardiomyocyte-like cells in the study of the mechanism of induction and autologous bone marrow mononuclear cell transplantation in the treatment of patients with acute myocardial infarc,R542.22
  18. Human MSC perfusion bioreactor and its biocompatibility of biomaterials,R318.0
  19. A Preliminary Study on Differentiation of Human Bone Mesenchymal Stem Cells into Human Corneal Epithelial Stem Cells,R329.2
  20. Preliminary Study on Treatment of Rabbit Cornea Injury by Human Bone Marrow Mesenchymal Stem Cells Carrying on Porcine Corneal Matrix,R772.2

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile