Dissertation > Excellent graduate degree dissertation topics show

The Role of Polarity Protein Scrib on Chick Gastrula

Author: XuTao
Tutor: WangLiJing；YangXueSong；GengJianGuo
School: Guangdong College of Pharmacy
Course: Pathogen Biology
Keywords: scrib numb gastrulation overexpression neural tube EMT
CLC: R321
Type: Master's thesis
Year: 2011
Downloads: 24
Quote: 0
Read: Download Dissertation

Abstract


Organogenesis and Differentiation initially occur in gastrula during embryo development. Blastula epiblast cells migrate to midline of embryo and undergo EMT to transit mesoderm in primitive streak. And then neural tube, somite, cardiac, brain, archenteron, vascular formed simitanously or in the follwoing. Many factors and genes were repoted to be involved in affecting cell migration in oogenesis and subsequent development, in which polarity genes are of importance in this process. The polarity protein Scrib is a scaffolding protein belonging to lap protein family. Knocking out scrib caused epithelial cells to lose apical-based polarity and abnormal cell cycle in drosophila. Human Scrib has been identified as a tumor suppressor gene. And scrib also play an important role in maintaining synapses function and internal ear development. Our laboratory previous research revealed that scrib can bind numb to negatively regulate notch signal in vitro experiments. But it unclear whether this signal play role on organism development. This subject aimed at overexpression of Scrib and numb gene to determine if early embryo develop normally or not in vivo using chick embryo model.We detected Scrib, Numb mRNAs expression in normal chick embryo by in situ hybridization. Scrib mainly express in anterior primitive streak during HH 4 embryo, and the expression is dispersed in HH7 along with embryo devlopment. Using the same experimental strategy, we obtained the uniform data in numb mRNA expression. After overexpressing scrib and numb by electroporation in HH 3-4 stages embryo, we found that the ability of sheet-like epiblast cells through EMT was inhibited in early gastrulation and postponed/deformed neurulation in later gastrulation. And we knock down Scrib which can promote neural tube development. Meanwhile we assessed some related proteins expression with neural development after manipulating gene expression level of Scrib or numb, and demonstrated that there was no effect on Pax-7 expression but inhibited expression of Islet-1.ConclusionScrib and numb genes are probably important in modulating chick development during gastrulation, in which more specifically scrib and numb could inhibit EMT process and neural tube closure during neurulation.

Related Dissertations

  1. Impact on Drosophila Brain Developement Through the Overexpression and Loss of Function of Gsα,Q75
  2. Cloning and Molecular Analysis of Lovastatin Biosynthesis Related Genes from Monascus Purpureus,TQ927
  3. Preparation of polyaniline nanostructures , characterization and biomedical applications,TB383.1
  4. Epithelial-Mesenchymal Transition (EMT) and the Expressive Effect of Emt on microRNAs in Lung Cancer A549 Cells,R734.2
  5. Study of Electromagnetic Tomography Image Reconstruction Based on Finite Element and Genetic Algorithm,TM15
  6. Homocysteine ??and its metabolic enzyme gene polymorphism and the relationship between neural tube defects,R748
  7. Study on the Expression and Signification of N-cadherin、bFGF、PCNA on the Neural Tube Defects Model in Golden Hamster Induced by Hyperthermia,R363
  8. Relationship of Endothelial Nitric Synthase Polymorphism and the Nural Tube Defects,R748
  9. Retrospective Analyzing the Change of Incidence of Neural Tube Defects in Hospital’s Newborn and Folic Acid Intervention in Chang Zhi Heping Hospital of Shanxi Province from 2000 to 2009,R174
  10. Congenital neural tube defects PLA_2, COX-2 expression and gene therapy,R714.5
  11. Expression of p38 Mitogen-Activated Protein Kinase and Bax/Bcl-2、 Caspase-3 in Neural Tube Defects Induced by Hyperthermia,R748
  12. Case-control Study on the Association between Periconceptional Dietary Factors and Neural Tube Defects,R151
  13. Mouse testis -specific miR-471 expression and function of a preliminary study,Q75
  14. Zebrafish ATOH8 Cloning, expression and preliminary functional studies,Q78
  15. Expression of NUMB and Its Significance in Patients with Hepatocellular Carcinoma,R735.7
  16. Study the Expression of P-JNK in Neural Tube Defects Induced by Hyperthermia,R748
  17. The Study of the Role of MAPKS in Golden Hamsters Neural Tube Defects,R748
  18. Application zebrafish affecting the function of neural tube defects in the PCP pathway genes,R748
  19. Philippine stress response in Arabidopsis gene function analysis of three related,X173
  20. Preliminary Studies on the Relationship of EMT and Its Related Factors with Metastasis of Colorectal Cancer,R735.34
  21. Cloning and Function Analysis of Pdpg1, an Endopolygalacturonase Gene from Penicillium Digitatum,S432.44

CLC: > Medicine, health > Basic Medical > Human morphology > Human embryology ( human embryology,embryology )
© 2012 www.DissertationTopic.Net  Mobile