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Objective: To study papaverine in rabbit cerebral vasospasm after subarachnoid hemorrhage in rats. MATERIALS AND METHODS: Unilateral carotid artery ligation secondary cisterna magna blood injection prepared rabbit model of subarachnoid hemorrhage, the choice of 72 healthy adult New Zealand rabbits were randomly divided into A, B, C, D 4 groups according to the design time Each different points of 24h, 72h, 7 days and 3 sub-groups, each sub-group 6. A group was sham-operated group, respectively, A1, A2, A3 represents sham feeding after 24h, 72h, 7 d subgroups group; B group SAH control group, respectively, B1, B2, B3 represents SAH model-making success After feeding 24h, 72h, 7 d subgroups groups; C group SAH PPA groups, respectively, C1, C2, C3 represents the intervention SAH PPA feeding 24h, 72h, 7 d subgroups group; D group gelatin SAH PPA sponges, respectively, D1, D2, D3 intervention on behalf of SAH PPA gelatin sponge after feeding 24h, 72h, 7 d subgroups groups, each feeding time at the corresponding point of the brains were removed at the optic chiasm 1.4cm, 2.0cm coronal sections were taken at HE staining, Bcl-2 and bax immunohistochemical staining immunohistochemical staining were observed in rabbits surviving hippocampal CA1 neuronal density and Bcl-2, bax expression. The experimental data were statistically analyzed using SPSS17.0 software. RESULTS: HE staining observed under light microscope, you can see Group A basilar artery vessel wall smooth, structural integrity, no thickening, B group vascular diameter stenosis, vascular endothelial cell swelling significantly, internal elastic lamina wrinkles, some endothelial cell loss, smooth muscle cell degeneration, C, D group vascular caliber mild stenosis, endothelial cell swelling slightly. Light microscope for HE staining hippocampal CA1 neuronal density counts, A group see scattered in neuronal apoptosis; B group showed a large number of hippocampal pyramidal cells; same point in time, C, D group in CA1 slices The number of apoptotic neurons less than B group. Each group Immunohistochemical staining observed results are shown: Bcl-2 and bax showed cytoplasmic staining positive cells were stained brown, and uniform diffuse staining. (1) Bcl-2 immunohistochemical staining neurons Observations: A group of hippocampal CA1 region in Bcl-2 staining shallow, while in B, C, D group, hippocampal CA1 region of Bcl-2 staining obvious, and compared to group B, C, D group more positive cells and staining deeper. (2) bax immunohistochemical staining neurons Observations: bax in hippocampal CA1 region A lighter staining, while in the B, C, D group, hippocampal CA1 bax staining obvious. B and C, D groups compared to the number of positive cells and staining more deeper. Statistical analysis showed that the survival of neuronal density between groups, Bcl-2 and bax expression intensity pairwise comparison there was a significant difference. Conclusion: 1.SAH enable hippocampal CA1 neurons in Bcl-2, bax expression increased. 2 papaverine (PPV) CVS after SAH has vasodilatory effects may be increased hippocampal CA1 neurons in the expression of Bcl-2, bax reduced expression of anti-apoptotic role to play. 3 papaverine (PPV) of sustained-release formulations in CVS after SAH vasodilator significant, but still not ideal gelatin sponge release carrier.
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