Dissertation > Excellent graduate degree dissertation topics show

The Study of Anti-caries Mechanisms of Aleppo Gall on Streptococcus Mutans

Author: LinJing
Tutor: ZhaoJin
School: Xinjiang Medical University
Course: Clinical Stomatology
Keywords: Aleppo Gall Streptococcus mutans GTF LDH
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 66
Quote: 0
Read: Download Dissertation

Abstract


Objective:Aleppo Gall is the main active ingredients extracted from Turkish galls, the mechanism of Aleppo Gall effected on oral bacterial is not clear. The aim of this paper is to study the inhibiting effect of Aleppo Gall on Streptococcus mutans in the state of planktonic and biofilm during the process of growth, acid metabolism and glycose metabolism; the effect of Aleppo Gall on lactate dehydrogenase (LDH) which is the main virulence factor lead to caries; on the activity of glucosyltransferase(GTF); and on the gene expression of gtfB、gtfC、gtfD. Then the paper also discusses the defence mechanism of Aleppo Gall on caries, provides theoretical and experimental basis for developing natural anti-caries preparation of Uighur. Methods:1) Slip diffusion method was used to measure the inhibition zone of each concentration of Aleppo Gall; two-fold dilution was used to measure Minimal inhibitory concentration (MIC) and Minimal bactericidal concentration (MBC) of Aleppo Gall effecting on the growth of Strepto-coccus mutans; The visible light semiquantitative method has been used to measure biomass (OD570) values so that the effect of medicine on biomass of oral bacteria biofilm has been discovered; 2) Using pH meter measureApH and Anthrone measured the effect of Aleppo Gall on acid ogenicity of Streptococcus mutans in different state and on intracellular polysaccharide; 3) Bradford method was used to measure the content of total protein of GTF,and anthrone method was used to measure gluean Polysaccharide. Assayed the activity of lactate dehydrogenase(LDH) with the pyruvate-dependent oxidation of NADH-with; 4)The gtfs mRNA levels under different condition were determined by real-time quantitative PCR. Results:1) Gallic tannins significantly inhibited the growth of Streptococcus mutans, MIC of gallic tanninson Streptococcus measured by dilution experiments mounted to 4mg/ml, MBC was 16mg/ml; the MBEC value of gallic tannin on the growth of single strain biofilm of Streptococcus mutans is 8mg/ml, which was measured by semi-quantitative determination of OD values in the condition of visible light; 2) Gallic tannins inhibited acid production of Streptococcus mutans and the synthesis of intracellular polysaccharide (WIG); 3) Results The activity of GTF and LDH in the state of biofilm was higher than planktonic; Aleppo Gall, NaF and Aleppo Gall union NaF inhibited GTF and LDH both in the state of planktonic and biofilm; LDH in the state of planktonic are more sensitive with drug. The activity of LDH was statistically significant in different culture conditions; the inhibition rate of Aleppo Gall is the highest, the secend one is NaF and the last one is Aleppo Gall union NaF; 4) Gallic tannins influenced the expression of Streptococcus mutans gtfs gene by different degrees. Conclusions:The effect of Aleppo gall and active components of Aleppo gall on oral Streptococcus mutans not only inhibits bacteria growth, acid metabolism and glycose metabolism, but also adjusts the expression of Streptococcus mutans gtfs gene, effectively prevent the occurrence of dental caries.

Related Dissertations

  1. Live alone the study of parasitic soup rabbit lumbar disc herniation model in vivo tumor necrosis factor in rats,R285.5
  2. The Molecular and Immune Mechanisms of the Mouse Liver and Spleen Damaged by Exposure to Nanoparticulate TiO2,R392.1
  3. Studies on the Protective Role of Uric Acid on 6-hydorxydopamine-induced Injury of PC12 Cells,R742.5
  4. Effect of Chitooligosaccharides on Streptococcus Mutans Biofilm,R780.2
  5. Study on Flame Retardation of PVA,TQ325.9
  6. Study on the MgAl13-LDH Dealing with High Fluoride Water,TU991.2
  7. The Study of the Serum KL-6 and LDH Levels and Influential Factors in Children with Viral Pneumonia,R725.6
  8. Study on Intravital Magnetic Targeted Specificity and Sustained Release Effect of Dextran-magnetic LDH-Fluorouracil Transshipment Model in Rats,R96
  9. A Clinical Study on the Treatment of Lumber Disc Herniation(LDH) by Percutaneous Laser Disc Decomprersion (PLDD) and HUOXUETONGLUO,R681.5
  10. 1. Effect of (–)-epigallocatechin-3-gallate (EGCG) on Amino Acid Transmitters Release after Oxygen-glucose Deprivation in Primary Cultured Cortical Neurons of Rats 2. Electrophysiology Research of L-type Cav1.2 Channel on HEK-293 Transfected Cell,R285
  11. The Preliminary Study on Growth and Biofilm Formation Ability of Streptococcus Mutans,R378
  12. The Value of Automatic DNA Image Cytometry and Multicriteria Detection in the Diagnosis of Pleural Effusion,R734
  13. SR-BI Suppresses Cytotoxicity-Induced by Trivalent Iron,R543.5
  14. Study on the Synthesis, Organic Intercalation and Photoluminescence of Eu-doped LDH,O621.2
  15. The Study of the Effect of Incubated Concentrations of Photosensitizer in HMME-Mediated Photodynamic Therapy on Anticaries,R781.1
  16. Study of Sulfurmetabolism Function for luxS Gene of the Streptococcus Mutans,R781.1
  17. The Clinical Research of Treating Lumbar Disc Herniations (LDH) with Integrated Percutaneous Laser Disc Decompression (PLDD) and Lumbar Vertebra Traction,R681.53
  18. The Prognostic Factors and Comparative Prognosis System of 212 Primary MDS Patients,R551.3
  19. Construction of the Markless htrA-Deficient Mutant, clp-P-Deficient Mutant and htuA-clpP-Deficient Mutant of Streptococcus Mutans with Cre-loxP System,R346
  20. Effects of Lemon Peel Extracts on the Glucosyltransferase Gene Transcription of Streptococcus Mutans,R285

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile