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Gossypol on human fibrosarcoma HT1080 cells apoptosis and its related mechanism

Author: LiuChaoZheng
Tutor: BaiJingPing
School: Xinjiang Medical University
Course: Surgery
Keywords: Gossypol HT1080 cells Bcl-2 Bax caspase-9 Caspase-3 Apoptosis
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 27
Quote: 0
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Abstract


Objective : To study the gossypol HT1080 human fibrosarcoma cell proliferation and induce apoptosis , inducing tumor cell apoptosis explore some of the relevant molecular mechanisms . Methods: In this study, human fibrosarcoma cell line HT1080 cells in vitro , the use of gossypol observe the impact of drugs on cell growth . Gossypol by MTT assay on HT1080 cells proliferation ; Hoechst33258, the role of electron microscopy after gossypol HT1080 morphological changes of apoptosis ; flow cytometry assay the role of HT1080 cells after gossypol early apoptosis rate ; Determination of gossypol before and after caspase-9, 3 activity changes ; Western blot was used to detect the role of HT1080 cells after gossypol on Bcl-2, Bax protein expression levels. Results: gossypol HT1080 human fibrosarcoma cell proliferation since inhibition, and this inhibition time and dose -effect relationship exists , namely the role of time and with the gossypol concentration increases, the inhibitory effect is more obvious. Gossypol treated HT1080 cell morphology changes , Hoechst33258 staining appeared after gossypol treatment, the high uptake of chromatin staining , after gossypol treatment, the tumor cells appeared electron microscope typical apoptotic morphology changed. Flow cytometry was used to gossypol has induced HT1080 cells of early apoptosis. HT1080 gossypol induced apoptosis process , has appeared Cacspase-9, Caspase-3 activation. Gossypol concentration-dependent manner down anti-apoptotic protein Bcl-2 expression , increased promotion of tumor cell apoptosis protein Bax . Conclusion: gossypol can inhibit HT1080 human fibrosarcoma cell proliferation and promotes apoptosis. Gossypol may by caspase-9, caspase-3 activation , downregulation of Bcl-2 protein , increased expression of Bax plays mitochondrial pathway inducing tumor cell apoptosis.

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