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The Research of Antiviral Activity of Weichang’an Pill on Rotavirus

Author: ZhangZuo
Tutor: LiXiaoMian
School: Tianjin Medical University
Course: Pathogen Biology
Keywords: Gastrointestinal Pills Rotavirus The thiazole Blue colorimetric analysis Serum pharmacology Suckling mice
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 40
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Abstract

Objective To investigate the gastrointestinal safety and pills in vitro and in vivo anti-rotavirus (Rotavirus, RV) SA11 strain effect. Studies the method 1 gastrointestinal Pills in vitro inhibition of RV effect: 1.1 using the tissue culture method to observe the cytopathic effect (Cytopathic effect, CPE) and the thiazolyl blue colorimetric analysis (MTT) detection of gastrointestinal safety and pills the Hengyuan monkey kidney cells ( MA104) of RV role. And evaluate the gastrointestinal safety and pills on the preventive effect of RV SA11 strain, the therapeutic effect and direct inactivation by different administration. 1.2 to use serum pharmacology prepared the gastrointestinal safety and pills containing serum rats after taking time containing serum to inhibit the effect of RV detected by observing the CPE and MTT method. 2 gastrointestinal safety and pills vivo suppression RV effect: observation suckling mice of the same age of 60, the same female rats fed suckling mice divided into different groups, to exclude breastfeeding may cause interference. 60 suckling mice were randomly divided into six groups, each group of 10 normal control group (A), the virus control group (B), ribavirin treatment control group (C group, that is, 10 times the commonly used clinical dose), gastrointestinal Pills high-dose group (D group, that is, 30 times the clinical dose), gastrointestinal safety and pills in dose group (E group, which is 20 times the clinical dose), gastrointestinal Pills low dose group (F, that is 10 times the clinical The usual dose). Without the virus orally top of Group A with 50μ1 MA104 cell culture medium gavage, the remaining five groups were activated with trypsin titer (TCID50) 10-6.3 of the virus suspension 50μl gavage, the inoculation natural sucking two days in a row. Infected 1h, A, B group treated with distilled water, the treatment group were given gastrointestinal Pills, ribavirin oral treatment, 20μl / 4 days in a row, the group did not give other medications were given ordinary feed and water. Clinical response observed in each group of suckling mice, growth and development, and detoxification. RV attack four days after random suckling mice were sacrificed five specimens of blood and small intestine specimens, five suckling mice continued to observe the clinical symptoms. Detected before and after the virus infected stool rotavirus antigen; heart blood was measured cytokines TNF-α, IFN-γ; spleens do T and B lymphocyte proliferation test; 4 days after infection of the small intestine HE staining to observe pathological changes of intestinal tissue observed suckling rat jejunum RV, antigen distribution; using immunohistochemical staining; using the Tunel cell apoptosis in situ detection The RV has caused intestinal apoptosis; of CPE titration neonatal rat small intestine in RV titer. Results gastrointestinal safety and pills vitro inhibition of the RV effect 1.1 Weichang'an pill prevention MA104 cells, therapeutic effect, direct inactivation. RV-50% inhibitory concentration (50% inhibiting concentration, IC50) were 0.261 mg / ml, 0.269 mg / ml, 0.114 mg / ml. Therapeutic index (Therapeutic index, TI) are 4.79,4.67,10.96. 1.2 containing gastrointestinal Pills serum dilution of 1:16 after administration 45min ~ 65 min, serum containing RV show better inhibition. Gastrointestinal safety and pills vivo inhibition the RV effect: TNF-α and IFN-γ value gastrointestinal safety and pills in dose group compared with the virus control group, the difference was statistically significant (P lt; 0.05); gastrointestinal Pills high-dose group T , B lymphocyte stimulation index compared with the virus control group, the difference was statistically significant (P lt; 0.05); HE staining showed gastrointestinal safety and pills can reduce the pathological changes of the neonatal rat small intestine; Immunohistochemistry showed gastrointestinal safety and pills can effectively suppress milk RV SA11 antigen expression in the rat small intestine; CPE titration neonatal rat small intestine in RV titer Show Weichang'an pill can reduce the titer of the role of the small intestine RV. Conclusions gastrointestinal Pills RV SA11 strain lesions occur in MA104 cells in vitro inhibition. 2 gastrointestinal safety and pills can be reduced in vivo RV-induced intestinal pathologic changes, the effective suppression of the small intestine of suckling mice RV SA11 antigen expression, and reduce the the RV titers role in the small intestine. The results show that the gastrointestinal safety and pills were inhibited in vitro and in vivo the role of the RV, and relieve the symptoms of RV infection theory experimental basis for the clinical application of parenteral treatment of Pills RV diarrhea.

The Research of Antiviral Activity of Weichang’an Pill on Rotavirus

Abstract

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