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Objective: To observe the dose of iodine herbal compound for experimental autoimmune thyroiditis (EAT) in serum thyroglobulin antibody (TgAb), thyroid peroxidase antibody (TpoAb determined by), interleukin-1 ,4,6 TNF-α, and the ultrastructure of the thyroid tissue, thyroid Pathomorphology thyroid cells apoptosis regulatory proteins of Fas / FasL, Bcl-2/Bax influence, and then filter out the best treatment AIT dose of iodine herbal compound, and to explore its mechanism from the multi-target. MATERIALS AND METHODS: Select the general level of female SD rats 75 adaptive feeding the following week, and then randomly selected 15 only for the normal control group, remaining as a model group. Second weeks of the control group given distilled water, to model drinking periodate water, the concentration of 500g / l. The fourth week of the model for primary immunization, applied by complete Freund's adjuvant (CFA) and TG (TG: CFA = 1:1) emulsified into water in oil like the foot double after subcutaneous multi-point injection twice, interval two days after injection of 100μg once per each rat. Incomplete Freund's adjuvant (IFA) emulsified TG (TG: IFA = 1:1) to be a multi-point injection by subcutaneous back in the fifth week, each time each injection of 100μg, and continuously around as strengthening the immune . Eight weeks into the mold. Will become the mode of rats were randomly divided into model control group (13), Chinese medicine treatment of low-dose group (14), traditional Chinese medicine treatment in dose group (14), Chinese medicine treatment of high-dose group (14). Grouping herbal compound after gavage 6 weeks of treatment, the rats were sacrificed, detect blood through the aorta using the following method: (1) enzyme-linked immunosorbent assay (ELISA): used to detect the serum of the rats immunohistochemical method in TgAb TpoAb determined by TNF-α, IL-1, 4,6 change (2): used to detect apoptosis in thyroid tissue of rats regulatory proteins Fas / FasL in and Bcl -2/Bax changes; bilateral thyroid (3) remove the rats, the application of electronic scales for weighing, observable weight of each group and analyzed statistically. (4) the pathological changes in the morphology of the thyroid tissue light microscope to observe the rats in each group; ultrastructural changes of thyroid tissue (5) the electron microscope to observe the rats in each group (6) SPSS13 · 0 statistical software, and meet the variance Homogeneity and normality using analysis of variance for comparison between the groups, and each group of data using the x ± s said, and the test results of each group were statistically analyzed. Results: Chinese medicine, in the low-dose group compared with model group: traditional Chinese medicine in the treatment groups, serum thyroid autoantibodies of TgAb, TpoAb and IL-1, 4,6, TNF-α level were significantly reduced thyroid tissue Fas / FasL, Bax expression decreased Bcl-2 expression in thyroid tissue increased thyroid weight in rats significantly reduced. Light microscope to observe the the thyroid treatment group organizational structure damage in model group. Electron microscope observation of thyroid tissue treatment group ultrastructural changes in model group. The above data, the traditional Chinese medicine, the role of the middle dose group were better than the low-dose group, and high, no significant difference between the medium dose group. Conclusion 1. The different doses Ruanjian gall soup has to reduce the role of EAT rat serum thyroid autoantibody levels and associated cytokine levels. 2. The different doses Ruanjian gall soup has a role to alleviate the the EAT the rat thyroid ultrafine pathological injury. 3. The different doses Ruanjian gall soup has a role to reduce the EAT rat thyroid pathological damage. 4. The different doses Ruanjian gall soup to reduce the role of EAT rat thyroid cells apoptosis. In the above experimental results, traditional Chinese medicine, the role of high-dose group were better than the low dose group, no significant difference between the medium dose group.
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