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Effects of Ursolic Acid on the Proliferation, Apoptosis and Cisplatin Sensitivity of Human Ovarian Cancer Cells

Author: FengLiPing
Tutor: YangXingSheng
School: Shandong University
Course: Obstetrics and Gynaecology
Keywords: Ursolic acid Ovarian Cancer Resistance to chemotherapy COC1 COC1/DDP Apoptosis Caspase -3
CLC: R737.31
Type: Master's thesis
Year: 2009
Downloads: 62
Quote: 1
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Abstract


Background: The drug resistance of tumor cells to platinum-based chemotherapy is a major obstacle in the treatment of ovarian cancer clinical. Abnormal apoptosis and anti-apoptotic key drug resistance and the development of one of the main reasons. Resistant tumor cell caspase -3 (Caspase-3) of the signal transduction pathway defects, so Caspase-3 activation was inhibited, eventually leading to decreased apoptosis. Ursolic acid (ursolic acid, UA) is a recent study found that the plant extract has a wide range of biological activity, its most prominent role in the anti-tumor. Has yet to see ursolic acid on ovarian cancer, especially the study of the effect of platinum-resistant ovarian cancer resistant ovarian cancer, its ability to increase sensitivity to cisplatin also no relevant reports. Objective: To investigate ursolic acid on human ovarian cancer cisplatin-sensitive cell lines C0C1 and cisplatin-resistant cell lines C0C1/DDP proliferation and apoptosis, and study its resistance reversal effect the C0C1/DDP cell lines to explore its possible mechanism. Methods: MTT assay with cisplatin or ursolic acid and cisplatin ursolic acid inhibition of proliferation of the C0C1 and C0C1/DDP cell lines joint; apoptosis rate of flow cytometry; live cell fluorescence staining techniques observe the morphological changes of cell apoptosis; Caspase-3 activity kit detection of apoptosis-related protein Caspase-3 activity; the immunochemical statutory detection of Caspase-3 protein expression. Results: Ursolic acid concentration in 5 ~ 40μmol / L of the two cell lines have significantly inhibit the proliferation in a time-and concentration-dependent; cisplatin 20μmol / L concentration of 1.25 ~ 10μg/mL Bear the AHA decreased survival of the cell lines in C0C1/DDP after ursolic acid significantly (P <0.05); 20μmol / L of the two cell lines apoptosis rate increased (P <0.05), the cells showed the typical morphology of apoptosis characteristics, Caspase-3 activity increased (P <0.05); cisplatin and ursolic joint apoptosis rate compared with the single cisplatin C0C1/DDP cell lines significantly increased (P <0.05), Caspase-3 activity increased ( P <0.05). Conclusion: Ursolic acid could significantly inhibit the proliferation of ovarian cancer the C0C1 and C0C1/DDP cell lines induced apoptosis and enhanced C0C1/DDP cell lines sensitivity to cisplatin-induced apoptosis and reversal of drug resistance mechanisms may related to the increase in Caspase-3 activity. Ursolic acid is expected to become a new drugs for the treatment of ovarian cancer, with first-line chemotherapy drug cisplatin can be combined to improve the efficacy of the latter is expected to to new coalition chemotherapy with cisplatin.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Ovarian tumors
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