Dissertation > Excellent graduate degree dissertation topics show
Effect of ARB on Expression of CD68 and MCP-1 in Adipose Tissue of Rats on Long-term High-fat-diet
Author: GuoCaiHong
Tutor: YuanLi
School: Huazhong University of Science and Technology
Course: Internal Medicine
Keywords: Obesity Adipose tissue CD68 MCP-1 ARB
CLC: R587.1
Type: Master's thesis
Year: 2008
Downloads: 64
Quote: 0
Read: Download Dissertation
Abstract
|
Objective: To observe the long-term high-fat feeding rat adipose tissue inflammatory changes and ARB (angiotensin II receptor blockers) drugs inhibit the RAS (renin-angiotensin-aldosterone system) infiltration of macrophages in adipose tissue and MCP 1 gene expression. Observed by blocking the RAS improve adipose inflammation and insulin resistance. Explore the relationship of fat inflammation and insulin resistance and ARB class of drugs to improve the possible mechanism of insulin resistance. Methods: Gao fat high-calorie diet in rats induced insulin resistance, valsartan eight weeks, hyperinsulinemic glucose clamp test for detection of changes of insulin sensitivity, immunohistochemical method, RT-PCR method for detection of adipose tissue CD68 (macrophage marker) and MCP-1 (monocyte chemotactic factor) protein and mRNA expression, and by radioimmunoassay, serum biochemical analysis of insulin, blood sugar, cholesterol and other metabolic markers. Results: obese rats abdominal obesity, visceral fat mass and fat tissue of inflammatory cells was compared with the control group was significantly increased (P lt; 0.01); clamp results showed that insulin sensitivity of fat group decreased significantly (P lt; 0.01), serum triglyceride (TG), free fatty acids (FFAs), fasting serum insulin (bins) levels were significantly higher (P lt; 0.01), the content of epididymal adipose tissue inflammatory cells compared with the control group significantly increased expression of CD68, MCP-1 mRNA is 2.6 times that of the control group, 2.1 times (P lt; 0.01) Valsartan after 8 weeks, compared with the high-fat group visceral fat content decreased (P lt; 0.01) the higher fat insulin sensitivity was significantly higher (P lt; 0.01), and significantly reduced the number of visceral adipose tissue inflammatory cells CD68, MCP-1 mRNA expression were 71% of the high-fat group, 73% (both P lt; 0.01) Immunohistochemical results showed that high-fat group compared with the normal group, CD68 protein content was significantly higher average luminosity of 75.24 ± 2.60 vs 35.89 ± 2.01 (P lt; 0.01), MCP-1 protein content was significantly increased, the average luminosity was 82.75 ± 0.99 vs 51.93 ± 1.73 (P lt; 0.01); intervention group compared with the high-fat group, CD68 protein content decreased, the average luminosity of 75.24 ± 2.60 vs 47.48 ± 2.33 (P lt; 0.01) , MCP-1 protein levels also decreased significantly, the average luminosity were 82.75 ± 0.99vs 55.82 ± 2.55 (P lt; 0.01). Compared with the high-fat group, the intervention group FFAs and TG levels have decreased, but the difference was not statistically significant (P gt; 0.05). Conclusion: Long-term high-fat high-calorie diet can make blood lipid levels and body weight of rats significantly increased RAS increased expression of inflammatory changes in visceral adipose tissue, insulin resistance rats. Increased adipose tissue expression of RAS, the generation of the adipose tissue inflammation and insulin resistance in a certain correlation exists. ARB-class drugs can significantly reduce the infiltration of macrophages in adipose tissue, and MCP-1 expression, and reduce inflammation, improve the state of insulin resistance in rats. Gao fat rats has obvious the Gao fat hyperlipidemia, valsartan no significant improvement of lipid metabolism disorders, but can reduce the fat content of animal organs.
|
Related Dissertations
- The Research Report about the Storing Quality of Jiro Persimmon Treated by 1-MCP Combined with Nano-Packaging,S665.2
- Analysis of Nanguo Pear Aroma Components and Its Variation during Storage,S661.2
- The Expression and Significance of Serum MIP-1β and MCP-1in Patients with Rheumatoid Athritis,R593.22
- Rhizoma Coptidis Inhibits LPS-induced MCP-1 Production in Murine Macrophages,R285
- MCP face electron reflection on the impact of image intensifier resolution,TN144
- Correlation between the Expression of High Mobility Group Box 1 and it Ligand Receptor for Advanced Glycation Endproducts in Lupus Nephritis,R593.242
- The Clinical Study with Exenatide in Patients of Overweight of Obesity with Type 2 Diabetes Mellitus Inadequately Controlled in Blood Glucose,R587.1
- Experimental Study on the Effect of Tubulointerstitial Injur of IGA Nephropathy in Rat with Shen-Yan-Zhi-Xue Pill,R285
- Semi-continuous function into outer space,O189.1
- Study on the Metabolic Characteristic of Physiological and Biochemical in Fresh-cut Carrot,TS255.3
- Effects of Maturity and 1-MCP Treatment on Fruit Quality and Browing of Banana-plum During Cold Stroage,S662.3
- Research on Expression of Chemokine MCP-1 and IL-8 in Peripheral Blood of Gastric Cancer Patients,R735.2
- Functional Evaluation of MCP-1 to Detect the Obstructive Hydronephrosis,R692.2
- Intelectin airway inflammation in asthma study the role of,R562.25
- Acupuncture expression of chemotactic factor-1 in myocardial tissue of rats of experimental diabetic cardiomyopathy,R245
- Treatment of cooling Xiaofengsan clinical observation and subacute eczema on IL-8, MCP-1 Preliminary impact,R275
- SLC30A8 and Monocyte Chemoattractant Protein 1 Polymorphism and Susceptibility to Type 2 Diabetes,R587.1
- The Influence of Different Protein Sources on Rumen Environment and MCP Content and Microbial Population Structure on the Northern Shannxi Villus Goat.,S827.5
- Study on Treatment Concentration of 1-MCP in Different Kiwifruit Cultivars,S663.4
- Effect of 1 - MCP and Propolis on Ualities of Fuji Apple Fruit,S661.1
- ’Yate’ Kiwi Fruit Electrical Properties of Post-harvest,S663.4
CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
© 2012 www.DissertationTopic.Net Mobile
|