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Background: Diabetes (diabetes mellitus, DM) is a common endocrine and metabolic diseases. With social progress and affluence of the people, and its incidence is growing up. Diabetes is difficult to cure, its major hazards in its complications, greatly weakened the quality of life of patients with diabetes. DM treatment programs is mainly control of hyperglycemia, including oral hypoglycemic agents, insulin and analogues. At home and abroad to develop a DM treatment of a wide variety of drugs, including insulin secretagogues (sulfonylurea and non-sulfonylurea) mode regulator of insulin secretion, insulin sensitizers, suppression of hepatic glucose production and release of glucose, inhibition of intestinal glucose formation and absorption, even the anti-DM drugs in combination with a multi-faceted synergies. These classic anti-DM can effectively control high blood sugar, but long-term use will have significant side effects. Therefore, the combination the DM pathogenesis explore new therapeutic targets is very necessary. Objective: To investigate the role of Zinc, chromium, selenium yeast complex anti-oxidation of trace elements (Yeast-Zinc, chromium, selenium united antioxidant micronutrients, YAM) intervention on oxidative stress status of experimental diabetic mice, cytokine expression, as well as the pancreas, liver pathological structures. Application of organic yeast composite antioxidant trace elements to provide a scientific basis for prevention and treatment of diabetes and its complications. Method: from 20 100 male Balb / c mice were randomly selected as a control group and negative control group (normal mice high doses of test substance), n = 10. The remaining 80 mice were given a one-time high-dose intraperitoneal injection of streptozotocin STZ (150 mg / kg) diabetic model. After the modeling, the diabetic mice, blood glucose levels were randomly divided into four groups, namely diabetic model group (DM), zinc-rich, chromium, selenium yeast Trace minerals and low (L) dose group, middle dose group (M) and high (H) dose group. After each dose group was given treatment 8 w eyeball blood, killed by cervical dislocation, separation of serum, to measure blood sugar, blood lipids, and serum superoxide dismutase (SOD) (superoxide dismutase, SOD), glutathione peroxidase (glutathione peroxidase, GPX), glutathione (glutathione, GSH), malondialdehyde (malondialdehyde, MDA), alanine aminotransferase (ALT) levels. Take the mouse liver, kidney, brain tissue, measuring tissue SOD, GPX, GSH, MDA content. Take part of the liver in mice and HE sections of the tail of the pancreas, the observed changes in the mouse liver and pancreatic pathology. Mice spleen homogenate, measured in the flow cytometer spleen lymphocyte IL-2, IL-4 The expression amount. Results: 1. Zinc-rich, chromium, selenium yeast complex trace elements can be significant in delaying the trend of rising blood sugar in diabetic mice, and in the high dose group was significantly (P lt; 0.05). Zinc, chromium, selenium yeast complex trace elements can reduce levels of diabetic mice serum TC, TG, LDL of HDL content (P lt; 0.05), show that the test substance is conducive to the recovery of the blood lipids in diabetic mice . Zinc, chromium, selenium yeast composite trace elements can reduce the content of MDA in the serum of diabetic mice increase of SOD, GPX, GSH content (P lt; 0.05), indicating that the test substance can alter the oxidative stress of diabetic mice status. Zinc, chromium, selenium yeast complex trace elements can reduce diabetic mice, liver, brain, kidney MDA content, increase of SOD, GPX, GSH content (P lt; 0.05), indicating that the test substance can be changed in diabetic mice important organs of the state of oxidative stress. Zinc, chromium, selenium yeast composite trace elements can ease the pancreas of diabetic mice, the pathological damage of the liver, indicating that the test substance can protect β-cells of diabetic mice. High doses of zinc-rich, chromium, selenium yeast complex trace elements of normal mouse liver ALT enzyme leakage rate of no effect, and can reduce the leakage rate of diabetic mice liver ALT enzyme, indicating that high doses of test substances on normal no damage of liver function, to combined liver biopsy test substance may have a protective effect in the liver of diabetic mice. Zinc, chromium, selenium yeast complex trace elements can improve diabetic mouse thymus and spleen index (P lt; 0.05), and increased spleen lymphocyte IL-2, IL-4 expression levels (P lt; 0.05), indicating that the test substance can reduce the inflammatory response of the diabetic mice to some extent, improve the immunity of the diabetic mice. Conclusion: The application of zinc-rich, chromium, selenium yeast composite trace elements can significantly delay the upward trend in blood glucose in diabetic mice, antagonistic diabetes oxidation invasion injury, ease the body's inflammatory response, improve immunity, and no high-dose trace elements of normal mice body injury. Prompted the test substance may be an important role to alleviate the symptoms of diabetes and its complications, and improve immunity.
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