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Objective: To observe the traditional Chinese medicine depolymerization Fushenning (JJFSN) diabetes mellitus (DM) rat renal tubular osteopontin (OPN), CD68 expression and renal protective effect . METHODS : STZ-induced diabetic SD rat model , will become the mode DM rats were randomly divided into four groups: model group , JJFSN group , irbesartan group , JJFSN irbesartan group and normal control group . Rats in each group using the appropriate interventions for 12 weeks . The conventional method of measuring the index of rat kidney , blood glucose, blood urea nitrogen ( BUN ) , serum creatinine ( Scr ) , urinary albumin excretion rate ( UAER ) ; HE staining of renal tissue pathological changes ; immunohistochemical assay tubular OPN , CD68 expression ; Western blotting detection OPN renal tissue protein expression . Results : The model rats fasting glucose of UAER in , BUN , Scr, kidney index was significantly higher than the normal control group ; kidney tissue OPN, CD68 expression was significantly higher than that in normal control group ; optical microscope, the tubular structure of abnormal changes significantly . The group indicators JJFSN group , irbesartan the group and JJFSN irbesartan were lower than the model group ( P lt; 0.01 ) , the renal pathological structural change compared with the model group ; JJFSN irbesartan group indicators significantly low in model group the , JJFSN group , and irbesartan group (P lt; 0.01), but did not reach the level of the normal control group . Conclusion : JJFSN can protect the the DM rat kidney morphology and function , the mechanism may be related with to reduce OPN content in kidney tissue down expression of CD68 , reduce inflammatory lesions of the kidney tissue .
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