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Depression is caused by a variety of reasons, the main symptoms of depression as a mood disorder or affective disorder. This disorder is a psychiatric common diseases, the incidence and prevalence is gradually growing. Complex mechanism of depression is generally believed that neurotransmitters, neuroendocrine. Currently, academia certainly 5 - hydroxytryptamine (5-serotonin ,5-HT) and acetylcholinesterase (Acetyl cholinesterase, AchE) participation is an important part of its incidence. The hippocampus is closely related to learning, memory, emotions, endocrine structure area, and there are a large number of 5-HT and AchE. Chinese medicine treatment of mental illness in China has a long history. Depression genus the medical depression syndrome, epilepsy and other areas, \In ancient literature, about 130 acupuncture points related to the treatment of mental illness, such as the four customs points, thirteen ghost points. Acupuncture (including electro-acupuncture) treatment of depression since the 1950s, observed over a long period of research, to obtain reliable results, has caused widespread concern at home and abroad. Acupuncture is a green therapy, without any side effects, only this is a great advantage compared to traditional antidepressants, the combined operation is simple, inexpensive, and easy for patients to accept. The study acupuncture treatment of depression mechanism to provide theoretical guidance for clinical, has a high value. Objective To observe the electro-acupuncture Baihui and Sanyinjiao hole depression hippocampal 5-HT and AchE expression, to explore the mechanism of electroacupuncture treatment of depression. Method healthy Wistar rats choose male, weighing 180-200 grams, open field test (Open-field test) scores similar to those of 35, 10 were randomly divided into control group (normal group), the model 25. Blank group without any stimulation, making modeling module with chronic unpredictable moderate-intensity stress law 21 days, open field test and sucrose consumption test (Volume of sucrose-intake test) to verify successful modeling. Then renumbered EA group and the model group were randomly divided into 11. EA group selected points Baihui Sanyinjiao frequency 2Hz, the current strength of 1mA continuous wave, line EA treatment 20min, 1 day, animals were sacrificed after 14 days, 5-HT and AchE in the area was determined by immunohistochemical method integrated optical density value (Integrated Option Density, IOD), and analyzed using Image-Pro Plus 5.0 image analysis software. Results of an open field test results of the number of horizontal movement (Crossing-movement times, Cm times): modeling the 21st-made module with the number of blank group reduced significantly different (p lt; 0.001) differences statistically significant. Made modules 14, 21, respectively, compared with before modeling were reduced, there are significant differences, a statistically significant difference (p lt; 0.01), and its number of horizontal movement with and reduce modeling time. Blank rats showed no significant change in the number of activities at different time points, no significant difference. The number of vertical movement (Rearing-movement times, Rm times): modeling the 21st-made modules and blank group compared to significantly reduce the number of significant differences (p lt; 0.001), with statistical significance. Made modules 14, 21, respectively, before modeling the number of comparisons to reduce differences and statistically significant (p-value lt; 0.01). The blank group exercise frequency did not change significantly in the different time points in rats, no significant difference. Sucrose consumption test results 21 days after modeling the blank group and the model group compared to sugar intake significantly increased, there is a significant difference (p lt; 0.001), statistically significant. Immunohistochemical results blank group, EA group and model group of 5-HT overall comparison, F = 8.00, p = 0.0017, the difference was statistically significant; alpha = 0.05 level, pairwise comparisons SNK method model group and the normal group and EA group differences were statistically significant, the difference of the normal group and EA group was not statistically significant. AchE overall comparison of the blank group, EA group and the model group, F = 3.68, p = 0.00375, the difference was statistically significant; alpha = 0.05 level, pairwise comparison, the model group and the normal group and electric SNK method acupuncture group differences were statistically significant, there is no statistically significant difference between the normal group and EA group. The conclusions of a number of horizontal movement of the open field test reaction activity of the animals, the number of vertical movement reflects explore the behavior of the animal and curiosity. After 21 days of chronic moderate-intensity stimulation, by analyzing the open the Ueno test data before and after the animal model, you can conclude that: the model rats there is a change in behavior, activity, explore the behavior and curiosity than in the control group rats reduced. The normal circumstances rat syrup have partial tropism. Sucrose intake in rats before and after contrast modeling can be seen: the interest of the model rats on sugar to reduce sugar preferences is far lower than the control group of rats are not any stimulus. Depression can cause a decrease in the hippocampus 5-HT and AchE acupuncture can improve experimental rat brain hippocampus 5-HT, AchE level repair damaged hippocampal neurons, stable structure, for therapeutic purposes, speculated that the electrical Governance treatment of depression may be the role of 5-HT, the activities of AchE produce.
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