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Objective: This project aims to TCM theory, under the guidance through clinical studies and animal experiments, observe BHF treatment of knee osteoarthritis clinical efficacy, and to explore its possible mechanism. Methods: Clinical study enrolled patients were randomly divided into BHF treatment group and celecoxib control group, 30 cases were treated with BHF and Celebrex capsules orally for 8 weeks, compare the clinical efficacy, TCM , symptoms and functional improvement. Experimental studies will be 8 weeks old SD rats were randomly divided into two groups, the Chinese Medicines five western medicine group 3, a day to give adequate water and food, adaptive feeding three days. Between animals and humans through the conversion dose, administered orally eight days, and then centrifuged blood serum was separated, stored backup. Milk rabbit chondrocytes were isolated and cultured and passaged with toluidine blue staining of cartilage cells exist, take the first two chondrocytes experiment, four kinds of matrix metalloproteinase levels were double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was measured absorbance values, and then converted into the actual concentration of the sample, were statistically analyzed. Results: Clinical Research: 1.WOMAC Osteoarthritis Index: After treatment, pain, joint stiffness, physical function, compared with their pre-treatment score improvement, the difference was statistically significant (P lt; 0.05); 2. In improvement of TCM, Chinese medicine group was 89.66%, western medicine group was 62.96%, the difference between the two groups was statistically significant (P lt; 0.05); 3. Disease Comparison of efficacy: After treatment, the disease was no statistical difference in efficacy compared significance (P gt; 0.05). Study: Different concentrations of Chinese herb medicine can lower levels of MMP-1, inhibition increased with the concentration increased, compared with the control group, there were significant differences (P lt; 0.05), celecoxib group containing serum group on the lower levels of MMP-1 is not obvious, and the control group showed no significant difference (P gt; 0.05); except 5% of the drug-containing serum and serum containing celecoxib groups did not significantly reduce the levels of MMP-3 outside the other three groups could reduce medicine containing serum levels of MMP-3, inhibition increased with increasing concentration, compared with the control group, there were significant differences (P lt; 0.05); different concentrations of serum containing traditional Chinese medicine and Western music Celebrex containing serum could reduce MMP-13 levels, compared with control group showed significant differences (P lt; 0.05); different concentrations of Chinese herb medicine and celecoxib containing serum were not significantly reduced TIMP-1 in content, compared with the control group showed no significant difference (P gt; 0.05); Conclusion: Clinical studies have shown that Celebrex in pain relief is better, while BHF improve function and improvement in TCM's effect better. Experimental studies have shown that BHF can inhibit matrix metalloproteinases MMP-1, MMP-3, MMP-13 production, inhibiting the destruction of cartilage matrix, thereby reducing the degradation and destruction of articular cartilage. Therefore, Bushenhuoxue side of the articular cartilage has played a role in the protection and restoration, and thus OA have a preventive effect.
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