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Objective: To observe the effect of different meridian points on the impact of the hemiplegic stroke spasticity rats PKA phosphorylation level and protein expression to explore acupuncture at different acupoints treatment of the disease mechanism for acupuncture treatment of spastic paralysis , relieve muscle The tension increased to provide experimental basis . Methods : the modified suture prepared rat MCAO model , reperfusion after 3 hours of use the Zea Longa 4 -point scale rating standard score of neurological behavior ; successful modeling three days after the start of treatment , treatment for 14 consecutive days , once a day ; the neurophysiological H reflex evaluation model and treatment ; PKA phosphorylation levels of serum using ELISA assay and Western blot method of rat brain stem and spinal cord protein expression . Results: 1 ipsilateral H - wave latency , yang acupuncture group compared with the model group was significantly longer , and the model group , Yin acupuncture group , yin and yang acupuncture group , baclofen group , MCPG group with significant difference ( P lt ; 0.01, P lt ; 0.05 ) ; ipsilateral H reflex restore the curves show yang acupuncture group H / M value up to 1.078 ± 0.1283, and the model group , overcast acupuncture group , yin and yang, the acupuncture group , the baclofen group of MCPG group , there were significant differences (P lt; 0.01, P lt; 0.05); 2, yang acupuncture group significantly increased serum , brain stem and spinal cord of PKA phosphorylation and protein level. expression , and the model group , Yin acupuncture group , yin and yang, the acupuncture group , baclofen group , MCPG group , there were significant differences ( P lt ; 0.01 , P lt ; 0.05 ) . Conclusion: acupuncture significantly inhibited the H-reflex hyperactivity and improve the recovery curve of the H-reflex inhibition of spinal motor neuron excitability ; 2 , acupuncture therapy to improve the hemiplegic stroke spasticity serum , brain stem and spinal cord of PKA phosphorylation the level of protein expression , the difference between groups was significant ; acupuncture treatment to relieve post - stroke hemiplegia spasticity , whose role is to yang strongest , followed by yin and yang by Yin and weakest .
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