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Food Safety Assessment of Genetically Modified Milk with Human Lactoferrin Gene

Author: WangYang
Tutor: LiuHongLiang
School: Tianjin Medical University
Course: Epidemiology and Health Statistics
Keywords: Genetically modified foods Turn human lactoferrin gene Recombinant human lactoferrin Substantial Equivalence Food safety assessment
CLC: R155.5
Type: Master's thesis
Year: 2011
Downloads: 74
Quote: 1
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Abstract


The purpose of this paper, the principle of \Exogenous expression of proteins toxicity and allergenicity turn human lactoferrin gene milk powder and its parental control milk powder, whole milk powder nutritional analysis, acute toxicity, subchronic toxicity, mutagenicity tests, evaluation transferred to milk ferritin gene cow milk powder of edible security nature, to explore the transfer genetically modified foods of application prospects provide a theoretical basis, create provide data, genetically modified foods and practical security evaluation system to establish systems of animals carry out exploration for genetically modified food database. Method 1 recombinant human lactoferrin acute oral toxicity test: GB15193.3-2003: 2 recombinant human lactoferrin in rats 28 days subacute oral toxicity test: \2004.5), new chemicals hazard assessment guide (HJ / T 154-2004): 3 recombinant human lactoferrin in vitro simulated gastric / intestinal fluid digestion stability test: the Ministry of Agriculture Bulletin No. 869; 4 into the lactoferrin gene Determination of cow milk nutrients: infant formula and milk powder - Determination of GB / T 5413-1997; the 5 turn human lactoferrin gene milk powder, acute oral toxicity test: GB15193.3-2003: 6 rpm human lactoferrin gene milk powder feeding study in rats 90 days: chemical test methods (SEPA 2004.5), \mouse sperm morphology test of lactoferrin gene milk powder: GB15193.7-2003: 8 turn human lactoferrin gene milk powder mouse bone marrow micronucleus test: GB15193.5-2003: turn human lactoferrin gene milk powder Ames test: GB15193.42003. The results of the acute oral toxicity of a recombinant human lactoferrin for female, male LD50 gt; 20g/kg · BW, is a non-toxic level; recombinant human lactoferrin 28-day sub-acute oral toxicity test in rats dose group increased urinary protein, no obvious toxic effects; 3 recombinant human lactoferrin 0-15s in simulated gastric digest all that the protein is easily digested in simulated gastric fluid does not have to digest the stability; stable in simulated intestinal fluid, indicating that the protein is not digestible; the 4 turn human lactoferrin gene milk powder parent control milk powder in protein, carbohydrates, fat, fiber, vitamins and minerals is still significant bands in simulated intestinal fluid other major nutrients, the difference was not statistically significant; the 5 turn human lactoferrin gene milk powder, acute oral toxicity, the male and female MTD gt; 20g/kg · BW, is a non-toxic level; 6 revolutions in human milk The ferritin gene milk powder and parental control powdered milk fed rats were 90 days in the animal body weight, food intake, food utilization, hematology indicators, the indicators of blood biochemistry, gross anatomy and histological examination, the difference did not statistically significant; 7 turn human lactoferrin gene milk powder and parental control milk powder mouse sperm abnormality test, the sperm malformation incidence difference was not statistically significant; mouse bone marrow micronucleus test, micronucleus incidence was not statistically significance; 9 Ames test results were negative, revertant no statistically significant differences in the number of colonies. Conclusion The results of this study show that: the toxicological evaluation of newly expressed substances, recombinant human lactoferrin no apparent toxic effects; allergenicity evaluation in simulated gastric digestion stability in simulated intestinal digestion stability ; analysis of the key ingredients, turn human lactoferrin gene major nutrients of milk powder with the parental control milk difference was not statistically significant; evaluation of food safety, the transfected human lactoferrin gene milk powder and parental control milk powder skilled malformation rate, the micronucleus incidence of revertant no statistically significant differences in the number of colonies, not yet turn human lactoferrin gene of milk powder on the health of the animals tested have a significant adverse effect.

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CLC: > Medicine, health > Preventive Medicine,Health > Nutrition, hygiene,food hygiene > Food hygiene and food inspection > Food hygiene and inspection
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