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Study of the Immunologic Protection on the Sarcoma-180 Bearing Mouse by Cartilage Polysaccharide

Author: CheWei
Tutor: LiuAnJun
School: Tianjin University of Science and Technology
Course: Of Food Science
Keywords: Cartilage polysaccharide The anti-tumor activity S180 sarcoma cells Cellular immunity Humoral immunity Cytokines
CLC: R73-3
Type: Master's thesis
Year: 2008
Downloads: 117
Quote: 0
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Abstract


Subject to short-chain polysaccharide extracted from pig cartilage, mouse sarcoma S180 cells were established animal ascites tumor model and solid tumor model to study its anti-tumor immune protection mechanism. S180 ascites tumor model, modeling 5 days after administration cartilage polysaccharide treatment research cartilage polysaccharide with mouse life extension rate relations, spleen index and thymus index changes. HE staining was used to observe the changes in the mouse spleen cells. Modeling after treatment and observation of cartilage polysaccharide on cellular immune S180 mice. MTT assay to examine cartilage polysaccharide S180 entity tumor-bearing mice immune system were measured ConA and LPS-stimulated the next mouse spleen cells and peripheral blood lymphocyte proliferation, found that T cells can be improved to varying degrees mice B cell stimulation index. The cytotoxic activity of NK cells in experimental and E rosette formation experiments showed that cartilage polysaccharides can enhance the activity of NK cells and NK cells in the spleen and peripheral blood mononuclear cells E rosette formation rate. Smear immunofluorescence detection of the spleen cells obtained transmembrane glycoprotein CD40 and CD40L expression level of a certain degree of upward. Flow cytometry (FCM) to detect changes in lymphocyte subsets, cartilage polysaccharide enables CD3 ~~ CD4 ~ cells and CD4 ~ / CD8 ~ cells were significantly increased to achieve adjust the proportion of lymphocyte subsets play adjust The role of the cellular immune function, immune regulatory function has been further improved. The macrophage phagocytosis experiments showed that cartilage polysaccharide can enhance macrophage phagocytic ability of neutral red and chicken erythrocytes, blood cells were stained cartilage polysaccharides can increase the number of peripheral blood neutrophils (PMN), which play the role of anti-tumor. The role of of cartilage polysaccharides of tumor-bearing mice humoral immune: unidirectional the agar diffusion immune experiment found that cartilage polysaccharide antibody IgG antibodies secreted by the tumor-bearing mice was significantly increased enhanced humoral immune effect, contribute to tumor immune. Determination of cytokines in peripheral blood of mice with ABC-ELISA method changes found cartilage polysaccharides can change the cytokine IL-2, IL-6, TNF-α secretion, regulating tumor development has a significant role in the treatment of cancer . In summary, through the study of cartilage polysaccharide on the S180 mice immune protective effect, prove cartilage polysaccharides have some anti-tumor activity, is a useful development potential anticancer activity of substances.

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