|
Objective To evaluate the erythropoietin (erythoropoietin, EPO) in the rat sciatic nerve injury model for nerve regeneration and functional recovery, also discussed EPO after peripheral nerve injury in rats neuroprotective mechanisms, and for clinical peripheral nerve injury treatment of experimental evidence. Methods 40 adult male SD rats were randomly divided into two groups, namely EPO group and nerve growth factor (nerve growth factor, NGF) group, each rat were removed at the bifurcation of the right sciatic nerve 10mm with silicone tube 10mm bridging sciatic nerve defect. EPO group and NGF group were injected erythropoietin (5,000 U / kg / day for 2 weeks) and nerve growth factor (2,000 units / kg / day for 2 weeks), administered immediately after surgery in both groups were administered two weeks. After 4 weeks and 8 weeks, 10 rats in each group were extracted for general observation, and functional learning, electrophysiological, AMD and histological observation, the above indicators to assess erythropoietin in the large rat sciatic nerve injury model of nerve regeneration and functional recovery. Results 1 after 8 weeks newborn rat sciatic nerve NGF group formed around a dense scar tissue, and the newborn rat sciatic EPO group to form a layer around only meager translucent film. (2) after 4 weeks of NGF group and EPO group sciatic function index (sciatic nerve fuction index, SFI) similar, no significant difference. SFI 8 weeks, both groups were significantly better than the four weeks when the SFI values ??and EPO group was significantly better than the SFI SFI values ??NGF group. 3 after 4 weeks, NGF group motor nerve conduction velocity (motor nerve conduction velocity, MNCV) was (9.20 ± 1.07) m / s, EPO group MNCV was (10.60 ± 1.36) m / s, MNCV values ??between the two groups The difference was statistically significant (P lt; 0.05). 8 weeks, NGF group MNCV was (16.37 ± 3.40) m / s, EPO group MNCV was (20.56 ± 4.18) m / s, NGF group MNCV values ??remained significantly lower than the EPO group. The application of erythropoietin significantly reduced motor nerve conduction velocity diminished. 4 Electron micrographs reveal a large number in the EPO group myelinated nerve fibers. In contrast, injection of NGF have fewer sciatic nerve myelinated nerve fibers. EPO group myelinated nerve fibers in the thickness of the myelin sheath and axon diameters were significantly better than the NGF group. 5 after 4 weeks and 8 weeks, both groups the number of myelinated nerve fibers were significantly different. Quantitative analysis revealed that the operative side of the sciatic nerve protein gene product 9. 5 (protein gene product 9. 5, PGP9.5) immunoreactive nerve fibers in the average optical density (mean optical density, MOD), and integrated optical density (integrated optical density, IOD) in the nerve at 4 weeks and 8 weeks were significantly increased, and the expression of PGP9.5 EPO group was significantly higher than NGF group. Conclusion Erythropoietin can promote peripheral nerve injury in rats after nerve regeneration and functional recovery, suggesting that erythropoietin treatment of human peripheral nerve injury potential clinical applications.
|