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Changes of Muncl-18 and Therapeutic Effect of Thyroid Hormone in Hippocampus of the Adult Hypothyroid Rats

Author: LiuChunLei
Tutor: ZhuDeFa
School: Anhui Medical University,
Course: Geriatrics
Keywords: Hypothyroidism Hippocampal Levothyroxine Munc-18
CLC: R581.2
Type: Master's thesis
Year: 2009
Downloads: 22
Quote: 0
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Abstract


Objective To observe adulthood hypothyroidism (referred to as hypothyroidism) rat dorsal hippocampus Munc-18 changes and hormone replacement therapy be able to make changes in protein levels return to normal. The method of 3-month-old healthy adult male Sprague-Dawley rats were randomly divided into: (1) hypothyroidism (PTU group) rats daily intraperitoneal injection of PTU 1mg/100gWB; (2) conventional dose replacement therapy group (PTU 5-T4 group) rats received daily intraperitoneal injection of PTU 1mg/100gWB four weeks after daily administration of L-T4 5μg/100g WB; (3) high-dose replacement therapy group (PTU 5-T4 group) rats daily intraperitoneal injection of PTU 1mg/100gWB surrounded given daily L-T4 20μg/100g WB; rats daily intraperitoneal injection of an equal volume of saline (4) normal control group (Control group). Modeling six weeks after, blood brain by radioimmunoassay detection in serum levels of T3, T4, immunohistochemical detection of the hypersensitivity SP method, optical micrograph of the hippocampal CA1, CA3 and DG subregions, biological image analysis system the CA1 initial layer, pyramidal layer, radiation layer, lacunar molecular layer, CA3 region starting layer, pyramidal layer, transparent layer, the radiation layer and the DG molecular layer, the gate region angle back Munc-18 immunoreactive products The optical density (OD) of the average optical density (average optical density, AOD) as observed. All data using single-factor analysis of variance (ANOVA). Results Compared with normal control group, hypothyroidism, serum T3, T4 significantly lower, respectively, for 53.6% of the control group, 77.5%; alternative to conventional dose treatment serum T3, T4 returned to normal levels; alternative to high-dose the therapeutic serum T3, T4 significant higher than the alternative treatment group and the normal control group. Immunohistochemical detection: Munc-18 immune response similar product in four groups of rat hippocampal CA1, CA3 and DG sub-area distribution, brown particles are distributed in the various sub-regions of each layer, but the positive particles different densities. The quantitative analysis showed that: Munc-18 in hypothyroid rats hippocampal CA3-PL, a significant reduction in the CA3-SR the DG-PL and DG-ML compared with normal control group; alternative to conventional dose treatment group, Munc-18 in CA3- PL and DG-PL compared with the normal control group was no significant difference in the CA3-SR and DG-ML was still significantly lower than the normal control group; Munc-18 in the high-dose alternative treated rats four control There was no statistically significant difference. Conclusion (1) hypothyroidism can cause Munc-18 expression in some regions of the adult rat dorsal hippocampus presentation layer specificity decreased. Munc-18 (2) hormone replacement therapy reduced back to normal, and there is a certain dose effect.

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Thyroid disease > Hypothyroidism
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