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Patients with rheumatoid arthritis in peripheral blood mononuclear cells TGF-β/Smad signal transduction pathway and its clinical significance of

Author: LiuChunYan
Tutor: WangLiWen
School: Nanjing University of Traditional Chinese Medicine
Course: Traditional Chinese Medicine
Keywords: RA TGF-β/Smad signal transduction pathway TGF-βR Ⅱ Smad4 Smad7 Smad4/Smad7
CLC: R593.22
Type: Master's thesis
Year: 2009
Downloads: 94
Quote: 0
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Abstract


Objective: To establish a detection of rheumatoid arthritis (RA) peripheral blood mononuclear cells (PBMC) in TGF-β/Smad (transforming growth factor -β/Smad) signal transduction pathway related factors in the expression levels of real-time quantitative PCR methods, evaluation TGF-β/Smad signal transduction pathway related factor expression in RA, and the disease occurrence, development, transfer and other aspects of normalization clinical significance; and explore its RA Syndrome Typing role in the pathogenesis of RA for the study and treatment provide new theoretical basis. Methods: A set of real-time fluorescence quantitative PCR (Nested-Real-Time-PCR) and general real-time fluorescence quantitative PCR (Conventional-Real-time-PCR) method to detect human PBMC TGF-βR Ⅱ, Smad4 and Smad7mRNA expression levels . Extraction in PBMC RNA, reverse transcribed into cDNA as a PCR template, according to the TGF-βR Ⅱ, Smad4 and Smad7 gene primers were designed using SYBR Green Ⅰ fluorescent dye incorporation for Real-Time-PCR, detection of human PBMC TGF-βR Ⅱ, Smad4 and Smad7 mRNA expression levels. Results: RA patients by detecting 58 cases with 29 cases of healthy people group in PBMC TGF-βR Ⅱ, Smad4, Smad7mRNA expression level and Smad4/Smad7 ratio, RA patient group in the TGF-βR Ⅱ, Smad4, Smad4/Smad7 ratio than the healthy population control group was significantly higher, the difference was statistically significant (P <0.01); Smad7mRNA expression level of healthy population control group, the difference was not statistically significant. The RA patients before and after treatment with different clinical stages and the TGF-βR Ⅱ, Smad4mRNA Smad4/Smad7 expression levels and ratios for comparison, RA active than inactive period after treatment than before treatment, the difference was statistically significant (P < 0.01); joint function in RA patients will be different classification TGF-βR Ⅱ, Smad4mRNA Smad4/Smad7 expression levels and ratios for comparison, Ⅲ grade higher than grade Ⅰ and Ⅱ, chopsticks significant difference (P <0.01), Ⅰ and Ⅱ level compared (P> 0.05), not statistically significant. In RA the TCM group comparison, TGF-βR Ⅱ, Smad4 and Smad7 mRNA expression levels did not differ between; but Smad4/Smad7 ratio on TCM grouping of some significance, in hot and humid, and liver and kidney deficiency and Blockage Phlegm dampness Blockage Blockage group and the comparison group, Smad4/Smad7 ratio increased, there is a significant difference. And related factors for correlation analysis, Smad4 and TGF-βR Ⅱ, Smad4/Smad7 ratio was positively correlated (r values ??were 0.262,0.268, P1 <0.05, P2 <0.05), TGF-βR Ⅱ and Smad4 in peak period were duration of 5-10 years. Conclusions: RA in PBMC of TGF-βR Ⅱ, Smad4mRNA expression levels and Smad4/Smad7 ratios than healthy population control group; active than inactive, higher than before treatment after treatment; RA patients with joint function grading, Ⅲ above the level higher than Ⅰ and Ⅱ stage; and found Smad4/Smad7 ratio on TCM grouping of some significance; its detection index can be used as a secondary diagnosis of RA and judgment of RA activity, prognosis and severity of RA joint injury indicators .

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CLC: > Medicine, health > Internal Medicine > Systemic disease > Autoimmune diseases > Autoimmune diseases, connective tissue disease > Rheumatoid arthritis
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