Dissertation > Excellent graduate degree dissertation topics show

The Effect of Adiponectin on p38MAPK and LPL in the Oleic Acid-induced Steatosis Hepatocyte

Author: ZhongXiLin
Tutor: ChenDongFeng
School: Third Military Medical University
Course: Internal Medicine
Keywords: nonalcoholic fatty liver disease (NAFLD) Adionectin steatosis hepatocyte model triglyceride p38mapk LPL oleic acid
CLC: R575
Type: Master's thesis
Year: 2008
Downloads: 198
Quote: 1
Read: Download Dissertation

Abstract


Background and ObjectiveNon-alcoholic fatty liver disease (NAFLD) is a common liver disease which refers a clinical syndrome with the steatosis of parenehymal liver cells and lipid storage in liver without heavy alcohol intake history. In the West, the incidence of NAFLD is about 20%-40%.In China,it is one of the common disease in recently 10 years.It is a important issue about the epitology, prevention and treatment of NAFLD. However,its pathogenesis has remained unclear. Insulin resistance (IR) is one of the important foundation of NAFLD formation.Moreover,adiponectin and its receptor is closely asscoiated with IR and glugose metabolism.The previous papers suggest that p38MAPK passway induced adiponectin leat to the process of the fat acid oxide and input glugose.Therefore, adiponectin plays an important role in the mechanism of NAFLD.Meanwhile,p38MAPK passway and LPL are the dominant factors in this process.So,we explore the effect and possible mechanism of adiponectin on the expression of p38mapk and LPL during the hepatosteatosis .MethodsHuman L-02 hepatocyte were randomly divided into 3 groups.①Group A: normal hepatocyte;②Group B: oleic acid-induced group, which incubated in complete medium and oleic acid;③Group C: oleic acid plus Adiponcetin group, which incubated in normal culture medium and oleic acid and Adiponcetin. A model of oleic acid-induced steatosis of human hepatocytes was established. The accumulation of lipid droplets in hepatocytes was observed by light microscopy after oil red-O staining. The expressions of LPL mRNA of each group was detected by RT-PCR. The expressions of p38mapk, Phosphor p38mapk ( p - p38mapk) and LPL proteins of each group was detected by Western blotting. The content of triglyceride (TG) in hepatocytes was also determined.by biological and chemical detection kit of each group .Results: 1. The model of oleic acid-induced steatosis of L-02 strain hepatocytes was established successfully. In control,after oil red-O staining ,it was not observed red lipid droplets in hepatocytes,distributing densely with clear cytoplasm and nuclei.While in group B,it was found a few steatotic hepatocytes in 24hr ,as well as massive red lipid droplets in the cytoplasm in 72hr ,compare with group B, steatositic hepatocytes was significantly decrease in group C.2. TG, ALT, AST of the group B had increasing tendency time-dependently. The value of TG, ALT, AST of each observing time among the NAFLD group were different significantly (P<0.01). TG, ALT, AST of the group B were higher than those of the control group at the 24hr, 48hr and 72hr(P<0.01). While,TG, ALT, AST of the group C were lower than those of the group B at the 48hr and 72hr(P<0.01).3. The expression of p38MAPK protein of each observing time among the three groups had not difference. For 72hr,the expression of p-p38MAPK protein was upregulated in groups B, and as compared 24hr,48hr. (P<0.01),while,the expression of p-p38MAPK was significantly decresed in groups C,and compared with that in the group B(P<0.01).4. LPL-mRNA in group C had decreasing tendency time-dependently. The value of LPL(LPL/β-actin)of each observing time among the group C were different significantly (P<0.05). In group B, LPL/β-actin ratio was lower than those of the group C of each observing time(P<0.01). The mRNA level of LPL in control group had not significant change.5. LPL protein level in group C had decreasing tendency time-dependently. The LPL protein level of each observing time among the group C were different significantly (P<0.05). In group B, LPL protein level was lower than those of the group C of each observing time(P<0.01). The protein level of LPL in control group had not significant change.Conclusions:1. Adiponectin can improve the hepatisteatosis in vrtio in some degree.2. Adiponcetin can significantly decrease the p-p38mapk protein expression and the levels of TG、ALT and AST in steatositic hepatocytes, however,upregulated LPL expression in steatositic hepatocytes.Thus,these results suggested that there was a significant role of Adiponectin in the development of non-alcoholic fatty liver disease.3. p38MAPK and LPL are the important factors of which Adiponcetin have participated in the signal transmission of hepatosteatosis.

Related Dissertations

  1. The Effects of Atorvastatin on Expression of Osteopontin in Kidney of the Diabetic Rats Its Molecular Mechanism,R587.2
  2. The Effect and Mechanism of p38MAPK Inhibitor CBS3830 on Intimal Hyperplasia in Autogenous Vein Grafts of Diabetic Rats,R587.1
  3. Early Responses of Bone Marrow Stem Cells to Different Mechanical Signals Through p38MAPK Signaling Pathway,R329
  4. The Study on the Regulation and Mechanism of Adipose Triglyceride Lipase by Fenofibrate and Pioglitazone,R96
  5. Relationship between Physical Condition and Correlative Factors of Middle-aged and Elderly People with Levels of Band 3 Protein of Qingdao,R195
  6. Effects of p38MAPK Signaling Pathway on Cyclic Stretch-induced Fibroblast Apoptosis,R781.4
  7. Expression and Correlation of Urokinase-type Plasminogen Activator (uPA) and Its Receptor (uPAR) and Mitogen Activated Protein Kinase (MAPK) p38 in Endometrial Carcinomas,R737.33
  8. Role of p38MAPK Signaling Pathways in the Apoptosis of C2C12 Myoblast Cells Subjected to Cyclical Stretch,R783.5
  9. Correlation Studies of Plasma Fatty Acids and Type 2 Diabetes Mellitus: Case Control Study,R587.1
  10. The Enzymatic Preparation Technology of Lard to Produce Human-milk Patterned Structured Triglyceride,TS221
  11. Study of Diacylglycerol’s Effect on the Blood Fat and Antioxidation of Rats,R965
  12. P38MAPK Signal Pathway Modulated Proliferation, Apoptosis and Cell Cycle of Rat Hepatic Stellate Cells Stimulated by Acetaldehyde,R575.2
  13. The Experimental Research about the Expression of JNK and p38 Apoptosis of Skeletal Cells in COPD Model Rat,R563.9
  14. Research of the Effect of Dietary High-cholesterol Seafood on Lipid Metabolism in Animals,TS254.1
  15. Solid superacid of SO_4 ~ ( 2 - ) / - of CeO_2 the preparation of ZrO_2 and biodiesel,TK6
  16. The Study of Tongsaimai on Adiponectin in the Model of Carotid Atherosclerotic Plaque in Rats,R543.4
  17. Study on Effects and Mechanism of Saponins of Sea Cucumber on Lipid Metabolism,R285
  18. Triptolide Induces Apoptosis of Hut102 Cells Through P38 MAPK Pathway,R285.5
  19. Role of P38 MAP Kinase Activation in Astragalus Polysaccharide-mediated γ-globin Gene Expression,R259
  20. The Enzymatic Synthesis of Triglycerides Containing n-3 Polyunsaturated Fatty Acids,TQ645.6
  21. Cloning and Analysis of Partial Lipid-related Genes from Large Yellow Croaker,S917.4

CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Liver and gall bladder disease
© 2012 www.DissertationTopic.Net  Mobile