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Limonin, synephrine and hesperidin, citrus fruit drop in natural active ingredients, a high value in the food and pharmaceutical industries. Composite extraction process of the central subject of this thesis, the active ingredient in citrus physiological fruit drop three different physiological fruit drop of citrus varieties twice our Songzi City, Hubei Province as the research object, for different varieties, different fruit drop period, different drying methods of the active ingredient under the citrus fruit drop is measured, the active ingredient were prepared by ethanol reflux extraction extract, rotary evaporated to a syrupy concentrate, and then using the different solubilities of the three active ingredients, sequentially separated, the extraction process Discussion and research. An active ingredient, the single extract citrus physiological fruit drop in the use of the color reaction and HPLC method of combining containing limonin preliminary evidence of the citrus physiological fruit drop, synephrine and hesperidin through the study, the following conclusions: kind of active ingredient. 2, the total extraction process using 50% ethanol reflux extraction, solid-liquid ratio of 1:50 (W / V), temperature 70 ℃, extracted three times, each time to extract The 2h Citrus physiological fruit drop three active ingredients best extraction process. The extraction rate: limonin 2.19μg/mg, Synephrine 4.85μg/mg, the hesperidin 24.47μg/mg, purity of 29.94%, 56.49% and 91.41%, respectively. 3 different pre-treatment of the active ingredients of citrus fruit drop, fruit drop in hesperidin content of 80 ℃ drying is greater than the other treatments (80 ℃ drying 40 ℃ drying, natural drying), lemon the bitter element content and synephrine content in 60 ℃ blast dried under a higher value. 4, between the content of the active ingredient contained in the two physiological fruit drop and different varieties of the same species, there are differences. Physiological fruit drop of the same species, second physiological fruit drop limonin content of hesperidin are greater than the first fruit drop, synephrine content decreased. The comparative analysis of the content of the main active ingredient in a different diameter citrus fruit drop diameter ≤ 1.5 cm physiological fruit drop extract Synephrine better second physiological fruit drop (diameter> 1.5cm) extract hesperidin, second physiological fruit drop (diameter> 2.5cm) is conducive to extract Limonin. 5 different varieties limonin content of orange (Peng Na, Newhall, lime) and pummelo varieties (Guanxi honey pomelo, grapefruit) relatively higher satsuma mandarin varieties, of which the highest Ban Na (3.52 ± 0.084μg/mg); the synephrine content relative orange satsuma mandarin varieties of the content of the National Day (Owari, No. 1) (Peng-Na, Newhall, lime) and pummelo varieties (Guanxi pomelo, grapefruit) higher the Owari highest (20.50 ± 0.89μg/mg); hesperidin are Guanxi pomelo highest grapefruit, wide leather citrus two varieties (National Day 1 and Owari) and two varieties of the sweet orange (Peng Na and Newhall) between the content does not change significantly.
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