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Studies on Adaptability of Over-summering in Chongqing and Establishment of Fast Propagation System of Briza Media Linn.

Author: LiChunPing
Tutor: LiMingYang
School: Southwestern University
Course: Floriculture
Keywords: Briza media Linn. Hot and drought tissue cultivation
CLC: S688.4
Type: Master's thesis
Year: 2010
Downloads: 29
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Abstract


Briza media Linn. is belong to Grarnineae and it is a perennial herb. In recent years, ornamental grass had been paid attention more and more by people,And had broad space for development and market prospects. Tn this paper, we took the introduced Briza media Linn. as the experimental materials,used the Fescue arundinacea Schreb. as comparision. We study on the adaptability of over-summering in chongqing region of the two ones,and establishment of fast propagation system of Briza media Linn..From this study, it can provide practical application and theoretical basis for guidance of the Briza media Linn. in Chongqing,but also provide a introduction and adaptation reference for the other ornamental grasses,and enriched ornamental plant resources in Chongqing.The main results are as follows:1. The adaptive research of summer for Briza media Linn.(1) Briza media Linn. and Fescue arundinacea Schreb. leaf which gone from natural green to curly yellow, but Briza media Linn. suffered hot damage symptoms is more badly than Fescue arundinacea Schreb..(2) The contents of chlorophyll in Briza media Linn. and Fescue arundinacea Schreb. leaf showed a trend of increased first and then decreased, but Briza media Linn. leaf chlorophyll content decline of time is earlier than Fescue arundinacea Schreb..(3) The content of MDA in Briza media Linn. and Fescue arundinacea Schreb. leaf retained rise. The content of MDA Briza media Linn. increased faster than Fescue arundinacea Schreb., and The content of MDA Briza media Linn. was higher than Fescue arundinacea Schreb..(4) The content of praline in Briza media Linn. and Fescue arundinacea Schreb. leaf was not obvious.(5) The content of the dissoluble protein in Briza media Linn. increased first and then decreased. The content of the dissoluble protein in Fescue arundinacea Schreb. leaf was not obvious.(6) Under the stress the activity of SOD, POD and CAT increased at first and then decreased. Briza media Linn. leaf of SOD, POD and CAT activity has dropped earlier than Fescue arundinacea Schreb., and Briza media Linn. leaf of SOD, POD and CAT activity decreased in the range of greater than Fescue arundinacea Schreb..(7) Summarise above we concluded that Briza media Linn. had weaker adaptation in Chongqing than Fescue arundinacea Schreb.. 2. Establishment of regeneration system of Briza media Linn.(1) The best explant for callus induction is mature seeds. It can be successfully established tissue culture system. The young stem was not able to induce callus.(2)The best basic medium for callus induction was MS. In the same hormone concentrations of MS and N6 medium, the rate of callus induction for MS medium was much higher than N6 medium.(3)The best medium for callus induction was MS+2,4-D 3.0mg/L+6-BA 0.3mg/L. The best subculture for callus induction was MS+2,4-D 2mg/L+6-BA 0.2mg/L.(4) The best medium for callus cytodifferentiation was MS+NAA 0.1mg/L+6-BA 1.0mg/L+KT2.0mg/L.(5) The best rooting medium was MS+NAA 0.2mg/L+AC 0.2g/L.

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CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Garden Plant Cultivation and application technology > Lawns and ground cover plants
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