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Ethanol Production from Tobacco Waste Using the Yeast Saccharomyces Cerevisiae

Author: XieLiPing
Tutor: ZhaoXiaoXiang
School: Donghua University
Course: Environmental Science
Keywords: Tobacco waste Ethanol Ferment Acid hydrolysis SSF fermentation Immobilized
CLC: S216.2
Type: Master's thesis
Year: 2010
Downloads: 88
Quote: 1
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Abstract


The development of the global economy and the scarcity of non-renewable resources, fuel ethanol as a biomass energy caused widespread concern. Traditional biomass ethanol is the use of the food by high-temperature cooking fermentation production, consuming large amounts of food and a lot of energy. However, in the case of the arable land is decreasing, the overall grain production decreased year by year, the growing rise in food prices, the use of biomass waste fermentation ethanol not only enables the utilization of waste resources, consistent with national non-grain ethanol industry and energy savings policy. The use of solid waste fermentation ethanol in recent years has been a hot research, many scholars use different biomass solid waste fermentation ethanol. This paper explores the use of tobacco waste fermentation ethanol, to explore its resource to provide new ideas and different fermentation process. Tobacco scraps the the acidolysis fermentation ethanol investigated the concentration of sulfuric acid (20% -60%), the ratio of solid to liquid (5% -20%), and time (20 min-180min), temperature (20 ° C -60 ° C). hydrolysis. By four factors and three levels orthogonal experiment and single factor experimentally derived optimum hydrolysis conditions: hydrolysis temperature of 50 ° C for 100min, solid-liquid ratio of 10% sulfuric acid concentration of 50%, the highest rate of hydrolysis. Tobacco waste in the best sulfuric acid under hydrolytic conditions, by five-fold dilution, and the pH value to 5-6. Learn from the filtered hydrolyzate (FH) with Saccharomyces cerevisiae (Sacchharomyces cerevisiae) fermented to produce ethanol, unfiltered the hydrolyzate (UFH, including hydrolysis residue) Add cellulase and Saccharomyces cerevisiae the (Sacchharomyces cerevisiae) fermentation. Diluted five times the filter part of the hydrolyzate fermentation ethanol largest ethanol concentration is 1.09 g / L, five times diluted unfiltered hydrolyzate 1.23 g / L. largest producer of ethanol the production was 54.5g/kg (tobacco) and 61.5 g / kg (tobacco). Preparation of ethanol in simultaneous saccharification and fermentation (SSF fermentation), examines the factors fermentation temperature (20 ° C to -35 ° C), the amount of strain was inoculated (5% -15%), the initial pH value (2.5-7.5), shake Bed speed (0,150 rmp / min) fermentation yield. The results determine the optimum conditions for the SSF fermentation of ethanol: fermentation temperature of 30 ° C, initial pH value of 3.5, the inoculation amount was 10%, and a shaker speed of 150 revolutions / min. Fermentation time, the the cellulase dosage and solid-liquid ratio of three as a variable yield of ethanol as a response value, yeast tobacco waste on ethanol production process mathematical model was established using response surface methodology (RSM). The experimental results and simulations have shown that the best process parameters: fermentation time of 68h, solid-liquid ratio of 5% and the amount of cellulase 30U / g. Composite immobilized fermentation studies through experiments that the three yeasts of a fixed ratio of single fixed yeast or both of two yeast has the advantage of fixed. On this basis, the use of orthogonal experiment to investigate the fermentation temperature (20-35 ° C), the filling factor of the immobilized beads (5-24%), and fermentation time (24-96h) and the initial pH value (3.5-6.5) ethanol yield. The results showed that the immobilized optimum fermentation conditions: fermentation temperature of 35 ° C, the ball filling ratio of 15%, the time of 72 hours, pH value of 3.5. By analysis of variance showed that these four factors are significant factors affecting the ethanol yield.

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