Dissertation > Excellent graduate degree dissertation topics show

The Effect of Fibronectin and Sonic Hedgehog and Bone Morphogenic Protein-2 Combined with Nano-Hydroxyapatite/Collagen for the Repair of Rat Femoral Bone Defects

Author: QianDong
Tutor: XuXiaoFeng
School: Jiangsu University
Course: Surgery
Keywords: marrow mesenchymal stem cells(mscs) bone morphogeneticprotein-2(bmp-2) sonichedgehog(shh) fibronectin(fn) nanoHydroxyapatite/collagen(nhac)
CLC: R318.0
Type: Master's thesis
Year: 2010
Downloads: 77
Quote: 0
Read: Download Dissertation

Abstract


Objective:To study the effect of Fibronectin(FN) and sonic hedgehog(shh) and bone morphogenic protein-2(BMP-2) combined with the compound of marrow mesenchymal stem cells (MSCs) and nano-hydroxyapatite/collagen(nHAC) bone on the treatment of rat femoral bone defects.Methods:MSCsobtained from SD rat bone marrow were cultured in four groups:control group:common nHAC;Group A: nHAC modified with BMP-2 and shh;Group B:nHAC modified with BMP-2 and FN;Group C:nHAC modified with FN and BMP-2.At different time points(3,6,9,12d),the numbers of cells proliferate were counted and the levels of alkaline phosphatase(ALP) activity were detected.The expression of osteoblastic phenotypes were demonstrated and collagen typeⅠimmunoblot were analyzed.Animal models of femoral bone defect were established in 30 Sprague-Dawley rats.They were divided into 3 groups randomly:Group a:the control group, defect was filled with nHAC;Group b:with nHAC/MSCs;Group C:BMP-2/FN/MSCs/nHAC.Environment scanning electron microscope(ESEM) observation was made 12 weeks after operation. Imaging and histological observation were made 3,6,9,12 weeks after operation.Results:After differentiation of MSCs obtained from SD.The osteoblast morphological characteristics,the osteoblastic phenotypes and collagen typeⅠprotein expresssion were changed at each groups,and the group C was most obvious being observed;The cell numbers and the expression of the ALP increased with cultivating time for the four groups and group C were most obvious being demonstrated.At the same time point compared group C with other groups,the difference had statistical significance(P＜0.05) All of 30 rats were involved in the result analysis.①No inflammation or rejection response was detectable during the experiment,and the wound healed well.②There were significant differences in bone regeneration between group C and the other groups in 3, 6,9 and 12 weeks after operation(P＜0.01).③Histomorphology test showed that group c had better osteogenesis ability than group b and group a;Group b had better osteogenesis ability than group a.Conclusions:The bone defect repair ability of tissue—engineering bone which constructed with FN/BMP-2/MSCs/nHAC is stronger than nHAC combined with MSCs or nHAC alone.It can well repair segmental bone defects,and is promising to be new implant material in bone tissue engineering.

Related Dissertations

  1. Lung revascularization reversal of experimental emphysema,R655
  2. The Study of the Change of Microenvironment in Colon and the Correlation of Laminin Gene and Ret Gene in Hirschsprung’s Disease,R726.5
  3. The Study of Bushen Mixture on Expression of Integrins α2, β1 and Fibronectin（FN） of Renal Interstitial Fibrosis Mice,R285.5
  4. The Effect of Hepatocyte Growth Factor Concentration on Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Cardiomyocyte-like Cells,R329
  5. The Experiment Study of the Promoting Reparative Effect of Bone Marrow Mesenchymal Stem Cells on the Rat Hepatic Ischemia-reperfusion Injury,R657.3
  6. Secondary Development Research of Baicalin and Demonstration Study on Safety Evaluation of Traditional Chinese Medicines Contained Aristolochic Acid,R285
  7. Of of Zuoguiwan vitro induced differentiation of MSCs transplantation in the treatment of mice with spastic cerebral palsy research,R742.3
  8. Budesonide synergistic induction of TGF-β in human bronchial epithelial cells and fetal lung fibroblasts fibronectin expression,R56
  9. Expression of Connective Tissue Growth Facor and Fibronectin in Epiretinal Membranes,R774.1
  10. The Preliminary Study on a Functional Peptide of FnBPA Protein Mediating Staphylococcus Aureus Invasion,R378
  11. Isolation, Culture and in Vitro Multipotential Differentiation of Mouse Marrow-derived Mesenchymal Stem Cells,Q813
  12. Preliminary Development of Tissue Engineering Ligament,R318.1
  13. The Dynamic Changes of the Plasma Fibronectin and Relationship between It and CT Scan Value in Neonatal with Hypoxic-ischemic Encephalopathy,R722.1
  14. Clinical Study on the Relationship between TCM Syndromes and the Contents Change of Serum, Urinary Fibronectin in Chronic Glomerulonephritis,R277.523
  15. Sublytic Complement C5b-9 Complex Induces the Proliferation of Glomerular Mesangial Cells and Secretion of Extracellular Matrix Associated with NF-κB Activation,R392
  16. The Experimental Study of the Effect of the Vacuum-assisted Closure on Activated Gelatinases and FN in Human Chronic Wound,R641
  17. Inductive Effects of Rhizoma Drynariac on the Human Dental Pulp Cells in Vitro,R285
  18. Effects of Different Concentrations of Peritoneal Dialysis Fluid on MCP-1 mRNA Expression and Secretion of CAPD Rat Peritoneal Mesothelial Cells,R459.5
  19. Study on Design of Stretch Equipment and the Effect of Stretch on Proliferation of Marrow Mesenchymal Stem Cells,R-331
  20. Effects of Shenqifuzheng liquid valsartan protective effect on the kidneys of diabetic rats,R587.1

CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > General issues
© 2012 www.DissertationTopic.Net  Mobile