Dissertation > Excellent graduate degree dissertation topics show

Mass Spectra Study on Micro Unknown Constituents and Degradation Products in Bulk Drugs

Author: WuJianMei
Tutor: PanYuanJiang；SunCuiRong
School: Zhejiang University
Course: Chemistry
Keywords: drug impurity HPLC/ESI-MS~n nuclear magnetic resonance (NMR) structural elucidation
CLC: R914
Type: Master's thesis
Year: 2010
Downloads: 191
Quote: 1
Read: Download Dissertation

Abstract


It is important to undertake qualitative analysis and quantitative analysis of drug impurities.High-performance liquid chromatography,when combined with electrospray ion trap mass spectrometry,possesses high separation efficiency and great qualitative capability.As it holds the advantages of high sensitivity,relatively short analysis time,low sample amount,and the ability to obtain structural information,HPLC/ESI-MS~n has been widely used as a powerful tool in various online drugs analysis.In this dissertation,the liquid chromatograpy/electospray tandem mass spectrometry(HPLC/ESI-MS) method was developed to identify the degradation products of crude drugs:Imipenem and Meropenem,also used to identify the impurities of Eprosartan and Levofloxacin carboxylic acid.The details are listed in the following:PartⅠA method based on HPLC/ESI-MS~n and nuclear magnetic resonance (NMR) has been established to analyze the degradation products of Imipenem and Meropenem in an acid solution,as well as the generation procedures involved.Offline Fourier transform ion cyclotron resonance mass spectrometry(FTICR-MS) was also performed to assign the accurate elemental compositions.The proposed structures by were confirmed by NMR technology.After conducting the tracing detection experiments,the structures of intermediate products in the acid degradation procedure were then identified.PartⅡThree competitive fragmentation methods of Eprosartan in the positive mode were summarized by electrospray mass spectrum multistage fragmentation experiments(ESI-MS~n).In this phase,HPLC/ESI-MS~n and NMR technologies were employed to identify the chemical structure of a microimpurity by comparing the spectral data of Eprosartan and its impurity.PartⅢThe chemical structure of the microimpurity in Levofloxacin carboxylic acid was identified by analyzing minor differences between Levofloxacin and the impurity in the NMR spectrometry.Furthermore,two competitive mass fragmentation methods in the positive mode were summed up by multistage mass fragmentation experiments.In addition,the recent progress of the LC/MS~n technique in pharmaceutical analysis was reviewed in brief.

Related Dissertations

  1. Cucumber Growth Promotion and Wilt Disease Suppression of Bacillus Subtilis SQR9 and Their Mechanisms,S436.421
  2. Stugy on Chemical Substance and Quality Standards for Two Traditional Chinese Medicines,R284.1
  3. Study of Purification and Analysis of Flavonoids in Ginkgo Biloba Leaf Extract,TQ461
  4. Study on Fluid Identification Based on Nmr CPMG and DE Pulse Sequence,P631.2
  5. Application of NMR Logging in Reservoir Classification in Hailaer Area,P631.2
  6. Studies on Effective Chemical Constituents and Pharmacological Action of Uygur Medicine Diaphragma Juglandis Fructus,R29
  7. Of Kaixinsan compound intestinal absorption of,R285
  8. Study on Clinical Pharmacokinetic and Bioequivalence of Olmesartan,R96
  9. The Bioequivalence Evaluation of Pentoxyverine Citrate Orally Disintegrating Tablet,R96
  10. The Mechanistic Researches of Sweet Recognition Based on an Artificial Sweet Tast Receptor Model,TS202.3
  11. Software System Design of Integrated NMR Spectrometer,O482.532
  12. Qualitative and Quantitative Analysis of Isodon Serra and Its Pharmacokinetics Study by HPLC-MS Technologies and the Fingerprint of Isodon Rubesens,R284
  13. Investigation of Chemometric Methods on Metabonomic Analusis by Using Nulear Magnetic Resonance Spectroscopys,Q591-3
  14. Detection Sensitivity and Optimization Techniques for Nuclear Magnetic Resonance Spectrometer,R445.2
  15. Studies on Alkaloids of Catharanthus Roesus,R284
  16. Study on the Constituents from Rabdosia Rubescens Hemsl.,R284
  17. Research and Application on XML-based Data Management Model for NMR,TP311.52
  18. Determination of Phthalates Residues by an ELISA Immunoassay and Instrumental Analysis,R114
  19. The Stauntoniae glycosides preliminary study on the proliferation of K562 cells,S567.239
  20. The seven endangered plants Sinopodophyllum chemical composition its resource study,R284

CLC: > Medicine, health > Pharmacy > Drug basic science > Medicinal Chemistry
© 2012 www.DissertationTopic.Net  Mobile