Dissertation > Excellent graduate degree dissertation topics show

Protective Effects and Mechanisms of Ischemic Postconditioning in Mice Corticostriatal Slices

Author: HePing
Tutor: ChenZhong
School: Zhejiang University
Course: Pharmacology
Keywords: Cerebral ischemia Ischemia postconditioning Oxygen deprivation Glucose deprivation Glutamate
CLC: R964
Type: Master's thesis
Year: 2010
Downloads: 55
Quote: 0
Read: Download Dissertation

Abstract


Cerebral ischemic postconditioning has emerged recently as a kind of endogenous strategies for neuroprotection, which was defined as a sub-threshold ischemic insult applied to the brain after ischemic reperfusion. It may have great clinical potential. However, protective mechanisms of ischemic postconditioning are largely unknown, especially the roles of glucose deprivation and oxygen deprivation as two factors in ischemia postconditioning has not been reported. Several lines of evidence imply that regulating the level of excitability neurotransmitter glutamate may be involved in ischemic postconditioning induced neuroprotection. Astrocytes play an important role in maintaining balance of extracellular glutamate by glutamate transporter(GLT-1) and glutamine synthetase (GS). Therefore, in present study we investigated the roles of glucose deprivation and oxygen deprivation in ischemic postconditioning, and whether the protection is associated with glutamate metabolism regulated by astrocytes. At first, we found that hypoxic postconditioning aggravated neurological function and increased infarct size after transient middle cerebral artery occlusion in mice. We also established a model of oxygen-glucose deprivation (OGD) postconditioning in mouse corticostriatal slices. Corticostriatal slices injury induced by OGD and reperfusion (OGD/R) was significantly inhibited by OGD postconditioning with 1 min of OGD treated at 5 min after 15 min of OGD. Furthermore, glucose deprivation postconditioning with 1 min of glucose deprivation treated at 5 min after 15min of OGD also produced significantly neuroprotection which is comparable with OGD postconditioning. However, oxygen deprivation postconditioning with the same procedure has no neuroprotection on OGD/R-induced injury. And OGD or glucose deprivation postconditioning significantly reversed the up-regulation of glutamate and down-regulation of GABA. They also remarkably reversed down-regulation of Glt-1 expression and GS activity induced by OGD/R. These results suggest that glucose deprivation in ischemic postconditioning is the main factor which induces neuroprotection. And this effect may be associated with up-regulating GLT-1 expression and GS activity in astrocytes and furthermore regulating glutamate and GABA levels, which probably inhibits neurotoxicity induced by ischemia reperfusion.

Related Dissertations

  1. Effects of Sulodexide on Focal Cerebral Ischemia-reperfusion Injure in Rats,R743.3
  2. The Effects of Astragaloside Ⅳ on the Neurocyte and the Expression of PBRs after Cerebral Ischemia-reperfusion Injury in Rats,R743.31
  3. The Expression of Caspase-9 after Focal Cerebral Ischemia-reperfusion Injury in Rats and Further Intervention of Minocyeline,R743.3
  4. Neuroprotection of Atorvastin in SD Rats with Focal Cerebral Ischemia,R743.3
  5. The Immune Inflammatory Response Early after Cerebral Ischemia Reperfusion in Mice and the Intervention of Albumin or Zinc Chloride,R743.3
  6. Early Changes of Hippocampal Metabolite in Animal Model of Alzheimer’s Disease Measured by 9.4T MRS,R749.16
  7. Expression and Distribution of Metabotropic Glutamate Receptor mGluR5 in Human Normal and Inflammatory Dental Pulp,R780.2
  8. Comparison of Susceptibility Weighted Imaging and Dynamic Susceptibility Contrast MRI in Patient with Acute Cerebral Ischemia,R445.2
  9. Effect of Gamma Globulin on NF-κB and MDA of the Brain Tissue Following the Focal Cerebral Ischemia-reperfusion in Rat,R965
  10. The Effects of Dl-butylphtalide on the Expressions of TRPM2 and EndG after Chronic Cerebral Ischemia in Aged Ras,R965
  11. The Effects of Minocycline on the Expression of NF-κB, ⅠκBα and TNF-α in Focal Cerebral Ischemia-Reperfusion Rats,R965
  12. Protective of Fasudil in White Matter of Rats Following Acute Cerebral Ischemia-reperfusion,R965
  13. Effect of Cromakalin on Microglia and iNOS in Rats Following Acute Cerebral Ischemia-reperfusion,R965
  14. Aerobic granular sludge rapid culture and Effect of monosodium glutamate wastewater treatment,X703
  15. Distribution of Glutamate-,GABA-,5-HT,ACh-and DA-containing Neurons in the Cerebral and Pedal Ganglia of Cristaria Plicata Leach,Q42
  16. Modification of Focal Cerebral Ischemia Model in Rats for Long Term Living,R245
  17. The Neuroprotective Effects of Angiogenesis Induced by Resveratrol Against Cerebral Ischemia Reperfusion Injury in Mice,R285.5
  18. Effect of Chaowei Buyang Huanwu Decoction on Notch1 and Its Ligand Delta1 in Rats after Focal Cerebral Ischemia,R285
  19. The Effect of Pentylmethylquercetin in Metabolic Syndrome Mice Due to Intervention of Monosodium Glutamate,R285.5
  20. The Protective Effect of Leonurine on Ischemic Stroke Rats and Its Action Mechanisms,R285.5
  21. The Effect of Peurarin Pretreatment on Endothelial Nitric Oxide Synthase in Brain Tissue and Protective Mechanisms of It on Brain in the Early Stage of Cerebral Ischemia in Rat,R285.5

CLC: > Medicine, health > Pharmacy > Pharmacology > Psychopharmacology
© 2012 www.DissertationTopic.Net  Mobile