Dissertation > Excellent graduate degree dissertation topics show

The Epidemiological Investigation and Inheritance Study of Mitochondrial DNA 12SrRNA A1555G Mutation in Yunnan Province, China

Author: MaTao
Tutor: XueXiJun；DaiPu
School: Kunming Medical College
Course: Department of Otolaryngology,
Keywords: sensorineural hearing loss heritage deafness mitochondria DNA mutaion 12SrRNA A1555G Preventaion
CLC: R764.43
Type: Master's thesis
Year: 2010
Downloads: 51
Quote: 0
Read: Download Dissertation

Abstract


Objective:To screen mitochondrial DNA 12SrRNA A1555G mutation, explore prevalence, inheritance, and drug intervention among sensorineural hearing loss group of patients in Yunnan, China based on the previous studies that have shown sensorineural deafness and mitochondrial DNA mutations is closely related.Methods:A total of 452 disseminated cases with sensorineural hearing loss were enrolled in this study. Briefly, it includes DNA extraction from peripheral blood, polymerase chain reaction(PCR) amplification, restriction enzyme digestion to detect the A1555G mutation positive individuals. For the positive individuals, a face to face interview or telephone questionnaire survey was preformed to detect the incidence of maternal family deaf and draw a detailed matriarchal family tree. Following that was the screening for family volunteer use DNA extracting, PCR amplification and sequencing for confirmation.Results:A total of 38 positive individuals with A1555G mutation were screened out of 452 cases with sensorineural hearing loss (8.4%). Altogether 10 familes out of 20 families with detailed family tress participated in the research:For those 88 people enrolled in the study,36 people are with normal hearing, but the rest 52 are with sensorineural deafness. However,84 out of those 88 cases had A1555G point mutation. And the sequencing test indicated 10 are heterogeneous while 70 are homogenous mutation. Except 12 patients had no aminoglycoside antibiotic medication history, all the rest cases have a history of aminoglycoside antibiotc medication.Conclusion:The proportion of patients with drug-induced deafness in high within Yunnan Province. And we also found a high mutation rate in mitochondrial DNA A1555G It is worthy to do DNA 12SrRNA A1555G mutation screening for druy intervention and prevention.

Related Dissertations

  1. Discovery and Mechanism Study of Mitochondrial Mild Uncoupler,R341
  2. The Research about the Effects of Overtraining and Dansheng Injection on Cardiac Muscle Apoptosis in Rats,G804.2
  3. Clone Cytoplasmic Male Sterile Nad Gene and Studies on Regeneration of Kenaf (Hibiscus Cannabinus L.),S563.5
  4. Experimental Study for Treating Autoimmune Sensorineural Hearing Loss with Injecting Immunological Regulation Gene in Inner Ear of Guinea Pig,R764
  5. The Effect of Complement Iron in Simulated Altitude Training on Mice’s Iron Metabolism and the Ativity of Respiratory Chain Complexes in Mitochondria from Lover,G808.1
  6. Investigation of SLC26A4 Gene in Patients Associated with Sensorineural Hearing Loss in Northwest China,R764.43
  7. Application of Cochlear Implantation in Deaf-Mutism with Otitis Media, LVAS, and White Matter Maldevelopment,R764
  8. Clinical Analysis of Bone Conduction Hearing Loss in Chronic Suppurative Otitis Media,R764
  9. Study of Auditory Characteristics and Diagnosis of Acute Low-frequency Sensorineural Hearing Loss,R764.43
  10. Systematic Review of Vasodilators for Sudden Sensorineural Hearing Loss,R764.43
  11. Research on sVCAM-1 Levels in SSHL,R764
  12. Effects of Matrine on the Oxidative Stress of Lungs Mitochondre in Pμlmonary Fibrosis Rats,R285.5
  13. Study the Effect on Energy Substances and Mitochondria Function of Salidroside for Periph Fatigue Rats’ Muscle Cells,R285.5
  14. Effects of N-n-buytl Haloperidol Liodide on Tranlocation of PKCε, α and Changes of Intracellular Calcium of Myocardial Cells in Rats after Hypoxia/Reoxygenation,R965
  15. Mitochondrial protein Mitofilin research in the pathogenesis of vitiligo,R758.41
  16. The Effect of Cold Autologous Blood Cardioplegic Solution to Energy Metabolism and Mitochondria of Immature Cardiocyte,R654.2
  17. IL-10 gene recombinant lentiviral vector into various channels topical treatment autoimmune inner ear deafness experimental study,R764
  18. Effects of Hydrogen Sulfide on Acute Myocardial Ischemia in Rats,R965
  19. The Study of Mitochondrial Toxicity in Vivo of Metacavir in Rhesus Monkeys,S859
  20. Fluorescent Nanoparticle-Based Mitochondrial Labeling and Cell Imaging,TB383.1
  21. Phylogenetic Analysis of Leymus (Poaceae: Triticeae) Inferred from Mitochondrial CoⅫ Sequence,Q949

CLC: > Medicine, health > Otorhinolaryngology > Otology,ear disease > Ear nervous system diseases > Deaf
© 2012 www.DissertationTopic.Net  Mobile