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Research Objective: Cataract is the same the blindness disease and oxidative damage induced cataract important factors. Melatonin (MLT) is an indole hormone secreted by the pineal gland, a human body effective free radical scavenging agents and antioxidants. The subject application melatonin in vitro oxidative damage in rat lens through in vitro antioxidant model experiments, testing their antioxidant capacity to explore the inhibitory effect of melatonin on hydrogen peroxide induced cataract formation, seeking more The ideal antioxidant drugs provide a theoretical basis. Methods: In vitro remove the normal development of the Wistar rat lens randomly divided into the following three groups: A group was the control group, B group hydrogen peroxide (H2O2) treatment group, C group in group B with different concentrations of MLT, MLT final concentration, respectively, for the group of 10 (C1), 30 (C2 group), 50 (C3 group), 100 (C4 group), 200 (C5 group) μmol / L. Cultured in vitro after 24h observed turbidity of the lens, the Image-ProPlus (IPP) 6.0 software to analyze the lens under the black bands mean optical density values; microplate determination of the protein content of the lens tissue, reduced glutathione glutathione (GSH), malonic aldehyde (MDA), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT) content. HE staining was observed under an inverted microscope lens epithelial cell morphology. Results: 1. Observation and quantitative analysis of the rat lens turbidity After incubation, the slit lamp visible group A lens remains transparent posterior gray line visible; B group lens full mixed white, gray lines blur posterior unclear; C group of lens transparency were higher than group B showed varying degrees of fuzzy gray line in the posterior. The average optical density analysis, the mean optical density value of the C group lens than in group B (P lt; 0.05). Group C results show that the C3 group and the the C4 group average optical density value is higher, the lens transparency, the difference between the groups was statistically significant (P gt; 0.05), the mean optical density value differences between other groups were statistically significance (P lt; 0.05). Rat lenses Determination of Biochemical end of culture the C rat lens tissue GSH, T-AOC, SOD, CAT, content is higher than in group B (P lt; 0.05) in group C, C3 group rat lens tissue GSH, T-AOC, CAT showed the highest activity (P lt; 0.05), the C3 group C4 SOD content difference was not statistically significant (P gt; 0.05). MDA content in rat lens tissue in group C than in group B (P lt; 0.05), C3, C4, C5 group group MDA content difference was not statistically significant (P gt; 0.05). 3 light microscope lens tissue cell morphology observed by HE staining, light microscope, blank control group anterior capsule continuous and smooth, the lens epithelial cells arranged in linear, nuclear elongated rod lens cortex fiber layer arranged in neat. Discontinuous the H202 treatment group anterior capsule lens epithelial cell proliferation, disorganized, part of the lens epithelial cells to the deep tissue migration, cells became larger, pleomorphic nuclei. Before MLT10μmol / L group lens capsule intact lens epithelial cell proliferation, the form of elongated rod-shaped, arranged in multi-layer, multi-located deeper level; MLT30μmol / L group, part of the lens epithelial cells, loss of the original monolayer arranged neatly crowding, arranged in neat lens cortex fiber layer; MLT50μmol / L of lens epithelial cells in a linear arrangement, the gap is more or less uniform, elongated rod-shaped nucleus, more consistent form. MLT increasing concentrations of visible lens epithelial cells in a linear arrangement, the gap is more consistent, and a small number of cells located deeper, lens cortical fibers arranged in layers close and regular, but MLT100μmol / L group vesicle formation. Conclusion: under the conditions exist in the H202 No MLT, all mixed white rat lens. Join MLT, under the conditions of the presence of H2O2, and the transparency of the rat lens H2O2 treatment group was significantly higher than that. MLT and its oxidized metabolites of multi-level has strong antioxidant effects against H2O2 oxidative damage to the lens. Added in the experimental rat lens GSH MLT cultured in vitro, T-AOC, SOD and CAT content higher than H2O2 treatment group, the MDA content below H2O2 treatment group, indicating that the MLT group of antioxidant capacity. MLT groups join from MLT 10μmol / L to MLT50μmol / L, with increasing concentration, the antioxidant capacity of the stronger, MLT at a concentration of 50μmol / L that is able to play an effective role. Light microscope rat lens epithelial cell morphology and the H2O2 treated anterior capsular incomplete, lens epithelial cell disorder, was multi-layered, cell gap increases, pleomorphic nuclei; while the in MLT group of epithelial cell damage than the H2O2 treated with light, MLT50μmol / L lens epithelial cells were single-layer linear arrangement, more uniform cell gap nucleus elongated rod-shaped form closer to the blank control group, the results of these experiments illustrate the MLT can increase oxidative damage to the lens of anti- oxidation ability to play a strong protective lens, the role of maintaining lens transparency.
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