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Epidermal Growth Factor Receptor Regulate CyclinE Expression in Pulmonary Adenocarcinoma and Squamous Cell Carcinoma Cell Lines

Author: LiuJingYi
Tutor: SongZuo
School: Kunming Medical College
Course: Oncology
Keywords: Lung cancer Epidermal Growth Factor Receptor Cyclin E
CLC: R734.2
Type: Master's thesis
Year: 2010
Downloads: 29
Quote: 0
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Abstract


Objective: the occurrence of the epidermal growth factor receptor with a variety of tumors closely related , however, many factors related pathway is far from clear . This study confirmed the epidermal growth factor receptor in non-small cell lung cancer cell lines provide experimental evidence for the regulation of the expression of cyclin E , to further clarify the non-small cell lung cancer with EGFR signaling pathway . Methods : ● Western blot and real-time quantitative PCR studies of lung adenocarcinoma SPC- A - 1, lung squamous cell carcinoma SK -MES -1 and three large cell lung cancer NCI-H661 cell lines expression of epidermal growth factor receptor ; ● RNA interference high expression of the epidermal growth factor receptor cell lines epidermal growth factor receptor were blocked Western blot detection of epidermal growth factor receptor and cyclin E expression levels change . Results : ● three cellular proteins and RNA sample volume is consistent , Western blot results show : the epidermal growth factor receptor in lung adenocarcinoma SPC - A - 1 and lung squamous cell carcinoma SK-MES-1 cell line expression , no expression in the NCI-H661 large cell lung cancer . Real-time quantitative PCR results show that : the epidermal growth factor receptor highly expressed in lung adenocarcinoma SPC - A - 1 and lung squamous cell carcinoma SK-MES-1 cell line NCI-H661 large cell lung cancer cell lines with low expression . ● RNA interference technology to block the epidermal growth factor receptor in squamous cell carcinoma and adenocarcinoma cell lines , Western blot results showed that the two cells , cyclin E expression were decreased . Conclusion: Epidermal growth factor receptor expression in lung adenocarcinoma SPC - A - 1 and lung squamous cell carcinoma SK-MES-1 cell lines do not express large cell lung cancer cell lines ; lung adenocarcinoma SPC-A-1 epidermal growth factor receptor in the regulation of the expression of cyclin E in lung squamous cell carcinoma SK-MES-1 cell lines .

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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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