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Chinese medicine prescriptions derived from long-term medical practice , is the crystallization of the wisdom of the Chinese nation and cultural treasures . How to develop safe , stable , effective and controllable modern medicine is the urgent need to address the key issues in the modernization of traditional Chinese medicine research . Therefore , the use of modern science and technology to study traditional Chinese medicine , and clarify the pharmacodynamic substances based on compatibility and mechanism of action , given the traditional medicine with modern scientific connotation , accelerated the process of modernization and internationalization of Chinese medicine is essential . In this study, specific research object to traditional prescription Shenmai square as the integrated use of chemistry, pharmacology , bioinformatics and molecular biology techniques , the study found that the effective component of Shenmai Fang and effective component compatibility , and from the molecular level elaborated the scientific nature of the party component compatibility . The main contents include: 1 . Component - based excluded method of Chinese medicine effective component identification method , applied to the the Shenmai prescription has effective components from the synergies of cyclophosphamide (CTX) chemotherapy S180 mice found . The results show that the panaxadiol saponins is the Shenmai Fangzhong CTX chemotherapy S180 mice synergies to effectively component , ginseng glycol type saponins , ginseng of calcitriol type saponins and Polysaccharide compatibility Shenmai square CTX chemotherapy S180 mice from the synergies compatibility . 2 . Using simplex lattice experimental design and multiple linear regression method , using rat myocardial ischemia model , ligation of the coronary left anterior descending artery observed a different set of square myocardial infarction area ( MIS ) , the activity of the serum , of Shenmai effective components compatibility optimize . The application results show that ginseng glycol type saponins , ginseng of calcitriol type saponins and Ophiopogonin ( 1:2:2 ) compatibility can reduce MIS, and serum enzyme activity has better efficacy . From the molecular level to elaborate the mechanism of action of anti-ischemic Shenmai effective components compatibility . The results show that after the the Shenmai effective component compatibility administration can improve symptoms of myocardial ischemia , has a protective effect on myocardial ischemia in rats and its protective effect and scavenging oxygen free radicals , and promote secretion of NO and the promotion of VEGF, bFGF , and PDGF expression promote angiogenesis related . Through the above research , this article basically clear Shenmai different parties in both anti-tumor and anti-ischemic efficacy material foundation .
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