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Ⅰ、Study on Purification of Silybin and Pharmacodynamics of Silymarin Solid Dispersion Ⅱ、Study on Molluscicidal Activity of JJ18 Inoculated to Beach Soil

Author: YangXun
Tutor: ChenJun
School: Jiangsu University
Course: Pharmacognosy
Keywords: Silibinin Recrystallization Solid dispersion Endophytic fungi Molluscicidal activity
CLC: R285
Type: Master's thesis
Year: 2010
Downloads: 149
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Abstract


This thesis consists of two parts: I silymarin Bin Purification and silymarin solid dispersion pharmacodynamic studies of silymarin (silymarin) is the Compositae water milk thistle (Silybum marianum L.Gaertn), total extraction of the active ingredients of the medicinal fruit matter, the main component of silibinin (silybin), the different silybin (isosilybin) of silymarin ning (silydianin,), milk thistle Pavilion (silychristin). Silymarin as a traditional hepatoprotective drug, because of its efficacy and an extremely low toxicity in the treatment of liver diseases has been widely used, has accumulated 30 years of clinical experience. In order to improve the bioavailability of silymarin, the papers prepared by solid dispersion and purified by recrystallization from research. The main contents and conclusions are as follows: (1) The milk thistle seed shell kernel separation, heat reflux extraction by refluxing of silymarin in milk thistle shell, single-factor test optimization optimum extraction conditions: ethyl acetate and The silymarin shell solid-liquid ratio = 1:6, extraction time of 8 h, extraction temperature of 78 ° C. Under these conditions, the heat reflux extraction the silymarin yield was 4.69%, heat reflux extraction four times silymarin yield of 6.18%. (2) prepared by the solvent method different proportions of silymarin-PVP solid dispersion apparent solubility and dissolution test inspection, select 1:7 proportion. The solid dispersions of silymarin apparent solubility in water is of silymarin 62 times, and 20 min after dissolution in artificial gastric juice, the dissolution rate of more than 80%. (3) the use of the CCl 4 induced acute liver injury model to examine the liver coefficient and alanine amino transferase (ALT), aspartate aminotransferase (AST), superoxide dismutase enzyme (SOD), malondialdehyde (MDA) content and tablets Yiganling, compared to a comprehensive study of each group with Hism function. The results showed that when silymarin PVP ratio of 1:7, solid dispersion the CCl 4 -induced acute liver injury in mice indicators have significantly improved, and the solid dispersion efficacy of high-dose group closest with the control group, indicating better than tablets Yiganling. (4) silymarin as a raw material, dissolved in ethanol at 80 ° C, 4 ° C refrigerator to stand overnight, silibinin re-crystallization trials. Integrated the silibinin purity and yield considerations, 70% ethanol for recrystallization solvent. At this point the silibinin the content of up to 97%. The the endophytic fungi Ⅱ JJ18 vaccination marshland soil molluscicidal activity preliminary study of endemic parasitic disease schistosomiasis is an ancient, serious harm to human health. Snails schistosome intermediate host the eliminate snails of great significance. Endophytic fungi in the money loose JJ18-based research group isolated from the stems of money loose Pseudolarix kaempferi Gord a good molluscicidal activity of the strains. To further study the the the JJ18 site MOLLUSCICIDAL feasibility of the study in the laboratory will be inoculated into the snail habitats JJ18 marshland soil cultivation and fermentation of the soil extract by detecting the molluscicidal activity of the fermentation broth determine survival after JJ18 inoculated soil, is designed to explore a new molluscicides route. The content and conclusions of this thesis are as follows: (1) endophytic fungi JJ18 liquid fermentation, fermentation products were freeze-dried, n-butanol extract, found that intracellular product of n-butanol extract antibacterial effect, and cell The outer product of n-butanol extract of E. coli and Staphylococcus aureus MIC 0.9 mg / mL and 1.35 mg / mL. (2) endophytic fungi JJ18 access snail habitats in marshland soil, under laboratory conditions for a period of time, select a different point in time was eluted with sterile water micro-organisms in the soil, and by examining the soil leaching solution fermentation products on snails role in the killing, in order to determine the survival of endophytic fungi JJ18 access to the soil. After the the JJ18 vaccination marshland soil molluscicidal activity of time-effect relationship test, the preliminary determination JJ18 Under the experimental conditions, in the marshland soil to survive at least 30 days. (3) through the Artemia acute toxicity test, it is found that compared with the the JJ18 access PDA the JJ18 vaccination sterilization 2h, after non-sterile soil, eluent acute toxicity of fermentation products decreased, which is certainly the effect of vaccination.

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