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Studies on Fingerprint of Lonicera Macranthoides Hand.-Mazz.

Author: WangYanYan
Tutor: WangYanHan；DengJun
School: Southwestern University
Course: Medicinal Resources Chemistry
Keywords: Macranthoides Lonicera confusa Chlorogenic acid Luteoloside Fingerprint HPLC method
CLC: R284.1
Type: Master's thesis
Year: 2010
Downloads: 165
Quote: 1
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Abstract


The macranthoides Caprifoliaceae Lonicera species in southwest China, southern China, is widely used as \distinction. Dried buds or open flowers with detoxification, cool wind-heat function, attending carbuncle swollen sore treatment, pharyngitis, erysipelas, toxic heat dysentery, common cold, febrile disease fever, widely used in traditional Chinese medicine prescription and medicines. Medicinal macranthoides a comprehensive system of quality control, the subject established the macranthoides herbs HPLC fingerprint and simultaneous determination of two kinds of active ingredient chlorogenic acid and luteolin glycosides content. The different batches macranthoides herbs chlorogenic acid and luteolin glycosides assay results show that different origin macranthoides herbs chlorogenic acid and luteolin content, should pay attention to choose the right planting geographical ecological environment and processing, planted on the sunny side of the 3-year-old first crop of herbs green bud bud is the best medicine-based sources, the best processing methods for fixing drying time after picking herbs not only contains more the active ingredients of chlorogenic acid and luteolin glycosides, and bright color, easy to save. The the optimized HPLC fingerprint of the chromatographic conditions the macranthoides herbs, selected Kromasil C18 column (4.6 mm × 250 mm, 5μm), acetonitrile (A) -0.1% phosphoric acid solution (B) gradient elution gradient program B :0-10 min 89%; 10 ~ 25 min89% -80%; 25 ~ 38 min 80% -77%: 38 ~ 57 min77% -71%; 57 ~ 60 min71% -89%, the flow rate of 1 mL / min detection wavelength of 238 nm, column temperature was 30 ° C. HPLC analysis of chlorogenic acid reference peak chromatogram similarity analysis to determine with 14 on the Xiushan macranthoides herbs, 30 batches and 13 batches of different origin macranthoides herbs Total peak macranthoides fingerprints. Lonicera confusa and honeysuckle under reproduces the botanical origin of the macranthoides calendar version of \disaggregated to provide a scientific basis, the proposed version Pharmacopoeia macranthoides from Lonicera confusa under sub-list and as an independent herbs. This experiment the macranthoides the chlorogenic acid and luteolin content determination method and fingerprint detection method methodological study results show that with good precision, stability, repeatability, and easy to operate, can used to control the quality of the macranthoides identify the authenticity of the herbs, provide a guarantee for its clinical efficacy.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
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