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Studies on Electrochemical Immunosensors Based on Nanomaterials for Carcinoembryonic Antigen and α-1-fetoprotein

Author: LiuYaXi
Tutor: YuanRuo
School: Southwestern University
Course: Analytical Chemistry
Keywords: Carcinoembryonic antigen Alpha-fetoprotein Gold Nanoparticles Chitosan Immunosensor
CLC: TP212.3
Type: Master's thesis
Year: 2010
Downloads: 142
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Abstract


Carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) is a commonly used tumor markers, an internationally recognized and is currently used in the diagnosis of many diseases such as lung cancer, pancreatic cancer, breast cancer, liver cancer and other auxiliary. Therefore, the detection of CEA or AFP has very important clinical value. Many immunoassay method for the determination of CEA or AFP is present, has been developed. The electrochemical immunoassay sensor has the advantages of simple preparation, testing speed, high sensitivity, low prices, convenient, in recent years, more and more key steps to detect CEA or AFP, while the production of electrochemical immunosensor is the use of simple, fast and effective method of biological molecules immobilized on the electrode surface. Using electrodeposition, electrostatic adsorption, covalent bonding, etc. constructed electrochemical immunosensor, and introduction of the gold nanoparticles, and its high specific surface area, adsorption capacity, can be fixed to the large number of antibodies on the electrode surface, the other having a good biological phase capacitive, can maintain the activity of the antibody. Thesis to carry out research work in the following areas: 1. Electrodeposition prepare chitosan-based - the study of the gold nanoparticle composite film detection of alpha-fetoprotein immunosensor proposed based on chitosan - nano gold composite film and thionine the prepared novel fetoprotein immunosensor. Electrodeposition chitosan - gold nanoparticles composite film modified glassy carbon electrode, re-use the glutaraldehyde to do a cross-linking agent will be the thionine fixed electrode, and then using thionine adsorbed gold nanoparticles fixed alpha-fetoprotein antibody . Electrode preparation process characterized by cyclic voltammetry, and the experimental conditions were optimized. Optimal experimental conditions, the immunosensor alpha-fetoprotein, cyclic voltammetry, the linear range of 0.40 ~ 200.0ng mL-1, the detection limit for 0.24ng · mL-1. The immune sensors using electrodeposition Preparation of Chitosan - gold nanoparticle composite films, compared with the traditional drop-coating method, the method is more conducive to the control chitosan - gold nanoparticle composite film thickness, the immune sensor has good reproducibility. sex. Carcinoembryonic antigen immune sensor based porous chitosan film and gold nanoparticles modified porous chitosan film modified glassy carbon electrode prepared by electrodeposition method, and then using glutaraldehyde electron mediator thionine covalently porous chitosan membrane crosslinked, and thus take advantage of thionine fixed gold nanoparticles at the surface of the electrode, and by the Gold Nanoparticle carcinoembryonic antibody, obtained current-carcinoembryonic antigen immune sensor. Investigated the surface morphology of the porous chitosan film by scanning electron microscopy, investigated through electrochemical impedance spectroscopy and cyclic voltammetry electrochemical properties of the electrodes, and a detailed study of the performance of the immune sensor. Experimental results show that the research work of porous chitosan film help to improve the fixed amount of the antibody, and ultimately improve the immune sensitivity of the sensor. Immunosensor for carcinoembryonic antigen detection range of 0.2 ~ 10.0 ng · mL-1 and 10.0 ~~ 160.0 ng · mL-1, a detection limit of 0.08 ng · mL-. In addition, the immune sensor has a good selectivity, good reproducibility and stability. SiO2 @ Au nanospheres labeled HRP and alpha-fetoprotein antibody labeled double antibody sandwich immunosensor developed based on HRP-SiO2 @ Au as the second antibody production of alpha-fetoprotein double antibody sandwich immunoassay sensor. Gold chloride acid modification of the surface of the glassy carbon electrode deposition layer of gold nanoparticles, gold nanoparticle strong adsorption capacity fixed alpha-fetoprotein antibody by double antibody sandwich immunoassay mode, the HRP-SiO2 @ Au-labeled secondary antibody coupled to the electrode on, for alpha-fetoprotein determination, response comparison with other methods, labeled secondary antibodies. Experimental results show that the method can be used in research work on the secondary antibodies in conjunction with a large number of HRP, so that the immune sensor sensitivity is improved. The immunosensor detection of alpha-fetoprotein 0.05 ~ 200 ng-mL-1, a detection limit of 0.019 ng · mL-1. And the immune sensor selectivity, good stability.

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Biological sensors,medical sensors
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