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Study on the Formaldehyde Microbial Biosensor

Author: TangHongYan
Tutor: CuiJianSheng
School: Hebei University of Science and Technology
Course: Environmental Engineering
Keywords: Formaldehyde Determine Microbial sensor Bacillus subtilis Bacillus licheniformis E. coli
CLC: TP212.3
Type: Master's thesis
Year: 2010
Downloads: 113
Quote: 0
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Abstract


Formaldehyde content has become an important part of today's living room, food, textiles, pollution monitoring safety indicators. Detection of formaldehyde spectrophotometric, electrochemical detection method, chromatography, and electrochemical sensors. Spectrophotometric method is limited by the operating conditions of the water bath, or concentrated sulfuric acid, etc.; electrochemical method is higher sample pretreatment requirements; chromatography equipment is relatively expensive, and the operation and maintenance is relatively cumbersome, inconvenient to use; the electrochemical sensor Disturbance substances, short life. Microbial sensor to measure formaldehyde screened strains formaldehyde response specificity determination of less interference, and faster detection speed because of its high sensitivity, good reproducibility, formaldehyde fast, intuitive The Determination good prospects. Topic Select Bacillus subtilis, Bacillus licheniformis, E. coli experimental strains prepared immobilized membrane polarographic dissolved oxygen electrode as a transducer of the microbial sensor to study the response of three strains of formaldehyde experiments subtilis Bacillus formaldehyde response is the most sensitive. Immobilized Bacillus subtilis strains, using the sandwich method for immobilization methods were studied, their immobilization. Opted immobilization carrier is diatomaceous earth, and to improve the response performance of the immobilized microbial membranes. And determine the the immobilized carrier added amount and the amount of bacteria, further optimization of the sandwich method fixed microbial technology. Through experiments to identify the best working conditions of the Bacillus subtilis microbial sensors, sensor performance test. The results show that the temperature sensor work best (33.5 ± 0.5) ℃, the test base solution in phosphate buffer solution pH 6.76, the optimal concentration of the base solution GGA 18 mg · L-1. Formaldehyde microbial sensor measurement standard curve was linear equation y = 13.534x-0.0177, linear correlation coefficient of 0.9966, the relative concentration of formaldehyde in the linear response range of 0.005 ~ 0.2 mg · mL-1. 10 times the parallel determination of the relative standard deviation of 1.13%, and the recovery was 107%. Interference test: the smaller phenol, methanol, and ethanol on the experimental determination of the interference, when the determination of 0.1 mg · mL-1 formaldehyde coexist phenol 450 times, 600 times of methanol, ethanol 2370 times. Acetaldehyde determination interference and determination of 0.05 mg · mL-1 formaldehyde, acetaldehyde, can coexist 10 times. The experimentally derived the immobilized biofilm save time more than 60 days. In order to verify the performance of of Bacillus subtilis formaldehyde microbial sensor, experimental select acetylacetone spectrophotometry sensor method for control experiments. Acetylacetone spectrophotometric measurement wavelength of 413 nm; boiling water bath time 3 min; chromogenic reagent dosage of 2.00 mL; measured color to cool for 5 min. Measured by the linear regression equation y = 0.0211x-0.0093, correlation coefficient of 0.9996. Microbial sensor while taking advantage of the research group filled with Bacillus subtilis and acetylacetone spectrophotometric method for the determination of the unknown concentration of formaldehyde. SPSS software on the measurement results of the two methods, the t-test, P gt; 0.05, the difference between the two is not significant, acetylacetone spectrophotometric the photometric microbial sensor measurement results are in good agreement.

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