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Preliminary Analysis of Transcriptional Regulatory Pathway of Tapetum Based on Microarray Data

Author: ZhangGuoQiang
Tutor: YangZhongNan
School: Shanghai Normal University
Course: Biochemistry and Molecular Biology
Keywords: Arabidopsis Tapetum development Gene regulation pathways Bioinformatics
CLC: Q943.2
Type: Master's thesis
Year: 2010
Downloads: 96
Quote: 0
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Abstract


Normal pollen formation , an important role in the normal development of the pollen from the plant reproductive process depends both on the the gametes molecular cell function , and also depends on the anther tapetal cells supply support . The model plant Arabidopsis tapetum development process is controlled by a complex series of gene regulatory networks , gene regulatory networks and specific genes and other genes in the relationship for the development of the molecular mechanism of in-depth study has important implications . Due to the important role of the tapetum in pollen development , there are already 5 tapetum development related transcription factor function has been reported . Preliminary work in our laboratory to determine the 5 upstream and downstream of the transcription factor relationship . But how the the the DYT1, TDF1, AMS, AtMYB103 four transcription factors regulating tapetum development , which several biological processes regulating tapetum development , unclear . Arabidopsis mutant dytl of tdfl ams ms188 inflorescence material microarray analysis , resulting in a high-throughput experimental data . Select the chip signal value ( mutant / wild-type ) lt; 0.5 gene as downregulated genes in different mutant backgrounds downregulated genes one by one according to the information of the the GO and Mapman database functional classification . After classification analysis , the down-regulated genes , can be divided into five functional categories: protein degradation , the RNA transcriptional regulation , cell wall synthesis and degradation kinase receptor and peptide transport . Tapetum development and function of transcriptional regulatory pathway , we found that DYT1 separate regulation of secondary metabolism , TDF1 regulate cell activities the AMS and AtMYB103 jointly regulate lipid metabolism . DYT1 the TDF1 , AMS, AtMYB103 , transcription factor common regulation tapetum development process is to affect the metabolism of protein , RNA regulation , cell wall degradation signal kinase receptor , peptide transport five functional modules .

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CLC: > Biological Sciences > Botany > Plant Cell Genetics > Plant Genetic Engineering
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