Dissertation > Excellent graduate degree dissertation topics show

Study on the Sensitivity of Melanin Synthesis of Magnaporthe Grisea to Tricyclazole in Heilongjiang Province

Author: ZhangXin
Tutor: JinXueHui
School: Heilongjiang Bayi Agricultural University
Course: Plant Pathology
Keywords: M. grisea Tricyclazole Melanin biosynthesis Resistant
CLC: S435.111.41
Type: Master's thesis
Year: 2010
Downloads: 65
Quote: 0
Read: Download Dissertation

Abstract


The trial tested Heilongjiang Province 116 strains of rice blast fungus growth and sporulation amount detection and quantitative determination of colony color observed and melanin, the results show that the ability of mycelial growth in Heilongjiang Province, the rice blast fungus strains sporulation capacity and ability to produce melanin there is a significant difference. The strains tested colony color lighter, produce less melanin concentration strains accounted for a larger proportion of Heilongjiang Province, the Blast Fungus Population melanin ability is not strong, but between regions within the region between strains and strains melanin ability to be very different. Further strain tricyclazole susceptibility testing shows that mycelial growth ability and sporulation ability of the strains with the strains regardless of the sensitivity of tricyclazole the strains produce melanin capacity and strain tricyclazole sensitivity, strains itself produces more melanin ability MIC-H values ??and the MIC-A value higher tricyclazole less sensitive. Strains of bacteria produce colony color and amount of melanin was highly significant positive correlation. Application of melanin the Dengqen A combination of germs melanin synthesis ability of quantitative analysis to determine the amount of germs melanin synthesis tricyclazole the sensitive baseline values ??(EC50-H value) 0.1935μg · mL-1. 83 tested strains tricyclazole sensitivity analysis is based on the sensitive baseline values, only measured three low-resistant isolates (3.61%), and other strains are susceptible strains of M. grisea in Heilongjiang Province tricyclic azole already low antibacterial strains appear, but the vast majority of strains still showed more sensitive tricyclazole Heilongjiang Province is still available as effective agents against Blast. Further tricyclazole inhibit mycelial melanization MIC-H and inhibit appressorium melanization Determination of MIC-A, the results show that the vast majority of strains MIC-H values ??in 0μg · mL-1 ~ 0.8μg · mL-1 between , MIC-H values ??between generations is stable; MIC-A value in a certain degree of volatility, but the sensitivity of different strains tricyclazole there is a big difference between generations. MIC-H values ??with MIC-A value was positively correlated with, but there are some differences in both variations. Rice regions of Heilongjiang Province M. grisea melanin biosynthesis there is a big difference in sensitivity to tricyclazole a big difference in the sensitivity of different strains of the same area or parcel isolated melanin biosynthesis tricyclazole The Magnaporthe grisea melanin biosynthesis description of each area in Heilongjiang Province the sensitivity tricyclazole is diverse. Colony diameter and sporulation of a low resistant isolates and susceptible strains were measured, and the results show that the performance of the the bacteria strains Tricyclazole sensitivity in melanin biosynthesis, ability to mycelial growth and sporulation ability is consistent. Low anti-bacterial strains and sensitive strains adaptability to growing conditions. The sensitive strain Tricyclazole neutral (pH 7) under the conditions more suited to the growth of low resistant strain is more suitable in a slightly alkaline (pH = 8) under the conditions of growth. The high sugar and high salt medium, low resistant isolates susceptible strains are more sensitive to high osmolarity. Potato medium more conducive to the growth of each strain and produce melanin, tomato juice medium more conducive to sporulation of various strains.

Related Dissertations

  1. Study of the Fabrics’ Thermal-Wet Comfort in Hingh Humidity Environment,TS101.923
  2. Proteomic Analysis of Vibrio Alginolyticus in Response to Four Types of Antibiotics,S941
  3. The Effect of RNA Interference-mediated ERCC1 Gene on the Chemo-treatment Sensitivity of NSCLC in Vitro,R734.2
  4. Drug Susceptibility Testing of Staphylococcus Aureus from Children Impetigo and Typing of Staphylococcus Aureus by Randomly Amplified Polymorphic DNA in Chengdu,R440
  5. The Relationship between ghrelin and High-fat Diet-induced Obesity in Rats,R589
  6. X12CrMoWVNbN ferritic heat-resistant steel thermal processing of Microstructure Evolution,TG142.73
  7. Water pump corrosion properties of metallic materials,TG174.3
  8. Broad-spectrum Resistance Gene Engineering Research Based on the Interaction of R Gene and Cognate Avirulence Gene,S511
  9. Functional Analysis of a Putative MgRhoGef1 Protein in Magnaporthe Oryzae,S435.111.41
  10. Expression of Chemerin in Liver of Diet Induced Type 2 Diabetic Rats with IL-6 Intervention,R587.1
  11. Antimicrobial Resistantce of Acinetobacter Baumannii Isolated from New and Old Endemic Areas in a Hospital and Molecular Epidemiological,R181.3
  12. Analyze Prognosis of Patients Infected by Carbapenem-Resistant Klebsiella Pneumoniae in ICU,R446.5
  13. On Preparation and Characteristic of Chestnut Resistant Starch,TS231
  14. Microstructures and Performance of Plasma Cladding High-Chromium Iron-Base Wear Resistant Coating by the Precursor Carbonization-Composition Process,TG174.4
  15. Containing the scandium aluminum-magnesium alloy cold-rolled deformation and annealing organization of and texture,TG146.22
  16. Turbine zinc alloy bearing materials and properties,TG166
  17. The Experimental Investigation and Nonlinear Finite Element Analysis of Z-Shaped Column’s Shearing Performance Subjected to Low-Cyclic Horizongtal Load,TU375.3
  18. Preliminary Study on the Clinical Observation & Some Mechanism of Action of the Qingwenbaidu Fluid of TCM in Treating HAP,R259
  19. In Vitro Antibacterial Activity of Constituent CP of Crataegus Pinnatifida Combined with β-Lactam Antibiotics Against Methicillin-resistant Staphylococcus Aureus,R96
  20. Studies on Chemical Constituents of Arnebia Euchroma and the Activity Against Multidrug-resistant Bacteria,R285
  21. Effect of Transgenic DREB3 Drought Resistant Soybean on Soil Enzyme Activity and Soil Functional Microorganism,S565.1

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Cereal crop pests and diseases > Rice pests and diseases > Disease > Transgression ( pass ) an infectious agent harmful > Rice blast
© 2012 www.DissertationTopic.Net  Mobile