Dissertation > Excellent graduate degree dissertation topics show

Molecular Pathogenesis Study of Charcot-Marie-Tooth 2L Caused by HSP22 Variation

Author: QinDaQiang
Tutor: LuoWei
School: Zhejiang University
Course: Neurology
Keywords: Charcot-Marie-Tooth disease heat shock protein 22 two-dimensional electrophoresis Proteomics
CLC: R741
Type: Master's thesis
Year: 2010
Downloads: 39
Quote: 0
Read: Download Dissertation


Background:Charcot-Marie-Tooth disease (CMT) is the most common inherited peripheral neuropathy presenting with the phenotype of a chronic progressive neuropathy affecting both the motor and sensory nerves and high morbidity. Electrophysiological studies and pathological basis of clinical electrophysiological or anatomical pathology findings distinguish two major types-the demyelinating form and the axonal form. CMT2L was found to be caused by a novel 423G->T (Lys141Asn) missense mutation of HSP22. The mechanism of the HSP22 variation is still unknown, there are two different theoretical speculation:1) the loss of function mutation,2) acquired the toxic effects. The relatively late onset of the disease, which suggests an accumulative tissue damage pattern, indicates a more indirect, perhaps protective role of the wild-type sHSPs rather than an immediate role in proper tissue function.Objective:To screen differentially expressed proteins in human sural nerve between CMT2L patients and normal controls for understanding the pathogenesis of CMT. Results:Six differential protein spots were identified by PDQUEST analysis. Six proteins were successfully identified by MS, which are Tubulin alpha-1B. Actin cytoplasmic 2、Tubulin alpha-1A、POTE ankyrin domain family member F、Glutathione S-transferase omega-1 and EH domain-containing protein 2. The last two proteins may be of significant implications in axon degeneration, and may have some correction with axon degeneration. They both co-localized with HSP22 in the cell cytoplasm.Conclusions:Glutathione S-transferase omega-1 and EH domain-containing protein 2 may be involved in the occurrence and development of CMT2L and the related mechanisms remain to be elucidated. Two-dimensional electrophoresis and mass spectrometry technique provide an effective technique for screening the differentially expressed proteins in the development and progression of CMT.

Related Dissertations

  1. The Gene Identification and Behavioral Analysis of CMT2L Type Transgenic Mouse Model,R746
  2. Study on Proteomics Diagnostic Model with Serum and Saliva of Thyroid Carcinoma in Combination of Disease and Syndrome,R736.1
  3. Screening and Identification of Serum Protein Associated with Chemotherapy Resistance/sensitivity in Non-mall Cell Lung Cancer,R734.2
  4. Proteomics-based Analysis of Low Abundance Proteins in Leaves of Wheat NILs Taichung 29*6/Yr5,S512.1
  5. Studies on Seed Dormancy of the Main Maize Inbred Line 08-641 in Southwest China,S513
  6. PrpJ1 and PrpJ2, Two Phosphatases in Anabaena Sp. PCC 7120: Their Roles in Oxidative Adaption and Substrates/Partners in Vivo,Q93
  7. Proteomic data mining method,TP311.13
  8. Icariin on rat osteoblasts secrete the impact of proteomics,R285
  9. Proteomic Analysis of Sut Melanocytes in Response to xCT Deficiency,R341
  10. Study Cerebrospinal Fluid Proteomic in Rats after Acute Spinal Cord Injury by SELDI-TOF-MS Technique,R651.2
  11. Abnormal Protein Expression and Modification in the Spinal Cord with ALS,R744.8
  12. Proteomic Study of Hippocampus and Cerebral Cortex in the Rats Model of Temporal Lobe Epilepsy,R742.1
  13. Quantitative Proteomic Study of Laser Capture Microdissection Purified Normal Human Bronchial Epithelia and Human Lung Squamous Carcinoma,R734.2
  14. Heat shock protein 60 in the left colon and right colon tissue Differential Expression,R735.35
  15. The Analysis and Identification of Proteins from Rhizome of Sonchus Brachyotus Dc. in the Corse of Dormancy by Two-dimensional Electrophoresis and Peptide Mass Fingerprinting,S451
  16. Physiological and Biochemical and Proteomics Preliminary Analysis of Ipomoea Cairica (Linn.) Sweet under Different Allelochemicals Stresses,S451
  17. Proteomic Analysis of Leaf Intercellular Fluid from Stripe Rust Resistance Wheat,S512.1
  18. Proteomics Analysis of Rice and Maize Chloroplast and Construction of Maize Chloroplast Transformation System,S513
  19. Differential Proteomics Analysis of the Zaosu Pear and Its Red Bud Mutation,S661.2
  20. Analysis of the Differential Proteomics between Fresh and Frozen-thawed Ram Spermatozoa,S826
  21. Identification of Immunogenic Associated Proteins of Mycoplasma Bovis by Two-Dimensional Electrophoresis Combined with Western Blot,S858.23

CLC: > Medicine, health > Neurology and psychiatry > Neurology
© 2012 www.DissertationTopic.Net  Mobile